Soot and house dust mite allergen cause eosinophilic laryngitis in an animal model

Peter C Belafsky, Janice Peake, Suzette M. Smiley-Jewell, Sunil P. Verma, James Dworkin-Valenti, Kent E Pinkerton

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives/Hypothesis: Chronic laryngitis (CL) is common and costly. One of the most common causes of CL is thought to be laryngopharyngeal reflux, although a significant percentage of individuals fail to get better with acid suppressive therapy. The role of other potential causes of CL such as allergy and environmental pollution has not been thoroughly investigated. Purpose: To evaluate the association between iron soot, house dust mite allergen (HDMA), and CL in an established animal model. Methods: Twenty-four guinea pigs were separated into four 6-week exposure groups: 1) saline (allergen control)+filtered air (pollution control); 2) HDMA (Dermatophygoides farinae)+filtered air; 3) saline+combustion particulates; or 4) HDMA+combustion particulates. The primary outcome measure was mean eosinophil profile (MEP) in glottic, subglottic, and trachea epithelium and submucosa. Results: The combination of iron soot and HDMA caused eosinophilia (elevated MEP) in the glottic (P<0.06), subglottic (P<0.05), and trachea (P<0.05) submucosa and epithelium (P<0.05). Conclusion: The combination of HDMA and iron soot resulted in laryngeal eosinophilia in an established guinea pig model of CL. The data support the notion that factors other than reflux may cause CL. Further investigation into eosinophilic laryngitis as a distinct clinical entity caused by exposure to environmental allergen and pollution is warranted.

Original languageEnglish (US)
JournalLaryngoscope
DOIs
StateAccepted/In press - 2015

Fingerprint

Laryngitis
Dermatophagoides Antigens
Soot
Animal Models
Environmental Pollution
Iron
Eosinophilia
Trachea
Tongue
Eosinophils
Allergens
Guinea Pigs
Epithelium
Laryngopharyngeal Reflux
Air Pollution
Hypersensitivity
Air
Outcome Assessment (Health Care)
Acids

Keywords

  • Allergy
  • Chronic laryngitis
  • Combustion particles
  • Environment
  • HDMA
  • House dust mite antigen
  • Iron soot
  • Laryngitis
  • Laryngopharyngeal reflux
  • LPR
  • Pollution
  • Soot

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Soot and house dust mite allergen cause eosinophilic laryngitis in an animal model. / Belafsky, Peter C; Peake, Janice; Smiley-Jewell, Suzette M.; Verma, Sunil P.; Dworkin-Valenti, James; Pinkerton, Kent E.

In: Laryngoscope, 2015.

Research output: Contribution to journalArticle

Belafsky, Peter C ; Peake, Janice ; Smiley-Jewell, Suzette M. ; Verma, Sunil P. ; Dworkin-Valenti, James ; Pinkerton, Kent E. / Soot and house dust mite allergen cause eosinophilic laryngitis in an animal model. In: Laryngoscope. 2015.
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AU - Pinkerton, Kent E

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N2 - Objectives/Hypothesis: Chronic laryngitis (CL) is common and costly. One of the most common causes of CL is thought to be laryngopharyngeal reflux, although a significant percentage of individuals fail to get better with acid suppressive therapy. The role of other potential causes of CL such as allergy and environmental pollution has not been thoroughly investigated. Purpose: To evaluate the association between iron soot, house dust mite allergen (HDMA), and CL in an established animal model. Methods: Twenty-four guinea pigs were separated into four 6-week exposure groups: 1) saline (allergen control)+filtered air (pollution control); 2) HDMA (Dermatophygoides farinae)+filtered air; 3) saline+combustion particulates; or 4) HDMA+combustion particulates. The primary outcome measure was mean eosinophil profile (MEP) in glottic, subglottic, and trachea epithelium and submucosa. Results: The combination of iron soot and HDMA caused eosinophilia (elevated MEP) in the glottic (P<0.06), subglottic (P<0.05), and trachea (P<0.05) submucosa and epithelium (P<0.05). Conclusion: The combination of HDMA and iron soot resulted in laryngeal eosinophilia in an established guinea pig model of CL. The data support the notion that factors other than reflux may cause CL. Further investigation into eosinophilic laryngitis as a distinct clinical entity caused by exposure to environmental allergen and pollution is warranted.

AB - Objectives/Hypothesis: Chronic laryngitis (CL) is common and costly. One of the most common causes of CL is thought to be laryngopharyngeal reflux, although a significant percentage of individuals fail to get better with acid suppressive therapy. The role of other potential causes of CL such as allergy and environmental pollution has not been thoroughly investigated. Purpose: To evaluate the association between iron soot, house dust mite allergen (HDMA), and CL in an established animal model. Methods: Twenty-four guinea pigs were separated into four 6-week exposure groups: 1) saline (allergen control)+filtered air (pollution control); 2) HDMA (Dermatophygoides farinae)+filtered air; 3) saline+combustion particulates; or 4) HDMA+combustion particulates. The primary outcome measure was mean eosinophil profile (MEP) in glottic, subglottic, and trachea epithelium and submucosa. Results: The combination of iron soot and HDMA caused eosinophilia (elevated MEP) in the glottic (P<0.06), subglottic (P<0.05), and trachea (P<0.05) submucosa and epithelium (P<0.05). Conclusion: The combination of HDMA and iron soot resulted in laryngeal eosinophilia in an established guinea pig model of CL. The data support the notion that factors other than reflux may cause CL. Further investigation into eosinophilic laryngitis as a distinct clinical entity caused by exposure to environmental allergen and pollution is warranted.

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KW - LPR

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