Sonic hedgehog signaling is decoded by calcium spike activity in the developing spinal cord

Yesser H. Belgacem, Laura N Borodinsky

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

Evolutionarily conserved hedgehog proteins orchestrate the patterning of embryonic tissues, and dysfunctions in their signaling can lead to tumorigenesis. In vertebrates, Sonic hedgehog (Shh) is essential for nervous system development, but the mechanisms underlying its action remain unclear. Early electrical activity is another developmental cue important for proliferation, migration, and differentiation of neurons. Here we demonstrate the interplay between Shh signaling and Ca2+ dynamics in the developing spinal cord. Ca2+ imaging of embryonic spinal cells shows that Shh acutely increases Ca2+ spike activity through activation of the Shh coreceptor Smoothened (Smo) in neurons. Smo recruits a heterotrimeric GTP-binding protein-dependent pathway and engages both intracellular Ca 2+ stores and Ca2+ influx. The dynamics of this signaling are manifested in synchronous Ca2+ spikes and inositol triphosphate transients apparent at the neuronal primary cilium. Interaction of Shh and electrical activity modulates neurotransmitter phenotype expression in spinal neurons. These results indicate that electrical activity and second-messenger signaling mediate Shh action in embryonic spinal neurons.

Original languageEnglish (US)
Pages (from-to)4482-4487
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number11
DOIs
StatePublished - Mar 15 2011

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Calcium Signaling
Hedgehogs
Spinal Cord
Neurons
Hedgehog Proteins
Heterotrimeric GTP-Binding Proteins
Cilia
Second Messenger Systems
Inositol
Nervous System
Cues
Neurotransmitter Agents
Vertebrates
Carcinogenesis
Phenotype

Keywords

  • G protein
  • Neuronal specification

ASJC Scopus subject areas

  • General

Cite this

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N2 - Evolutionarily conserved hedgehog proteins orchestrate the patterning of embryonic tissues, and dysfunctions in their signaling can lead to tumorigenesis. In vertebrates, Sonic hedgehog (Shh) is essential for nervous system development, but the mechanisms underlying its action remain unclear. Early electrical activity is another developmental cue important for proliferation, migration, and differentiation of neurons. Here we demonstrate the interplay between Shh signaling and Ca2+ dynamics in the developing spinal cord. Ca2+ imaging of embryonic spinal cells shows that Shh acutely increases Ca2+ spike activity through activation of the Shh coreceptor Smoothened (Smo) in neurons. Smo recruits a heterotrimeric GTP-binding protein-dependent pathway and engages both intracellular Ca 2+ stores and Ca2+ influx. The dynamics of this signaling are manifested in synchronous Ca2+ spikes and inositol triphosphate transients apparent at the neuronal primary cilium. Interaction of Shh and electrical activity modulates neurotransmitter phenotype expression in spinal neurons. These results indicate that electrical activity and second-messenger signaling mediate Shh action in embryonic spinal neurons.

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