Abstract
The ciliary marginal zone (CMZ) has long been known to be a source of postembryonic neuronal production in the retinas of fish and amphibians, and more recently, birds. However, there is little known about the factors that are required for the maintenance of this neural stem cell zone. The cells of the CMZ respond to mitogens such as endothelial growth factor, insulin-like growth factor-1, and insulin, factors that are also mitogenic for embryonic retinal progenitors, suggesting that the continued expression of embryonic mitogenic factors might be required to maintain the postembryonic proliferative potential of the CMZ. To test this hypothesis, we examined the expression and functional role of a critical embryonic retinal progenitor mitogen, Sonic hedgehog (Shh) in the regulation of proliferation of the cells of the CMZ. We have found that Shh is concentrated at the retinal margin of postembryonic chicks. Moreover, we report that intraocular injection of Shh stimulates proliferation of the CMZ cells, whereas cyclopamine, an inhibitor of the Shh pathway, inhibits CMZ proliferation. We conclude that Shh signaling is an important factor in the maintenance of postembryonic retinal neurogenesis.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 66-75 |
| Number of pages | 10 |
| Journal | Developmental Dynamics |
| Volume | 233 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 2005 |
| Externally published | Yes |
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Keywords
- Neurogenesis
- Regeneration
- Retina
- Stem cells
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Sonic hedgehog regulates proliferation of the retinal ciliary marginal zone in posthatch chicks. / Moshiri, Ala; McGuire, Christopher R.; Reh, Thomas A.
In: Developmental Dynamics, Vol. 233, No. 1, 05.2005, p. 66-75.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Sonic hedgehog regulates proliferation of the retinal ciliary marginal zone in posthatch chicks
AU - Moshiri, Ala
AU - McGuire, Christopher R.
AU - Reh, Thomas A.
PY - 2005/5
Y1 - 2005/5
N2 - The ciliary marginal zone (CMZ) has long been known to be a source of postembryonic neuronal production in the retinas of fish and amphibians, and more recently, birds. However, there is little known about the factors that are required for the maintenance of this neural stem cell zone. The cells of the CMZ respond to mitogens such as endothelial growth factor, insulin-like growth factor-1, and insulin, factors that are also mitogenic for embryonic retinal progenitors, suggesting that the continued expression of embryonic mitogenic factors might be required to maintain the postembryonic proliferative potential of the CMZ. To test this hypothesis, we examined the expression and functional role of a critical embryonic retinal progenitor mitogen, Sonic hedgehog (Shh) in the regulation of proliferation of the cells of the CMZ. We have found that Shh is concentrated at the retinal margin of postembryonic chicks. Moreover, we report that intraocular injection of Shh stimulates proliferation of the CMZ cells, whereas cyclopamine, an inhibitor of the Shh pathway, inhibits CMZ proliferation. We conclude that Shh signaling is an important factor in the maintenance of postembryonic retinal neurogenesis.
AB - The ciliary marginal zone (CMZ) has long been known to be a source of postembryonic neuronal production in the retinas of fish and amphibians, and more recently, birds. However, there is little known about the factors that are required for the maintenance of this neural stem cell zone. The cells of the CMZ respond to mitogens such as endothelial growth factor, insulin-like growth factor-1, and insulin, factors that are also mitogenic for embryonic retinal progenitors, suggesting that the continued expression of embryonic mitogenic factors might be required to maintain the postembryonic proliferative potential of the CMZ. To test this hypothesis, we examined the expression and functional role of a critical embryonic retinal progenitor mitogen, Sonic hedgehog (Shh) in the regulation of proliferation of the cells of the CMZ. We have found that Shh is concentrated at the retinal margin of postembryonic chicks. Moreover, we report that intraocular injection of Shh stimulates proliferation of the CMZ cells, whereas cyclopamine, an inhibitor of the Shh pathway, inhibits CMZ proliferation. We conclude that Shh signaling is an important factor in the maintenance of postembryonic retinal neurogenesis.
KW - Neurogenesis
KW - Regeneration
KW - Retina
KW - Stem cells
UR - http://www.scopus.com/inward/record.url?scp=17444391316&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=17444391316&partnerID=8YFLogxK
U2 - 10.1002/dvdy.20299
DO - 10.1002/dvdy.20299
M3 - Article
VL - 233
SP - 66
EP - 75
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 1
ER -