Abstract
The influence of somatostatin on discrete stages of collagenous-matrix-induced endochondral bone formation has been investigated. Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75% reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. The minimum effective inhibitory dose of somatostatin was 0.25 μg/day. Twice daily local injections of the hormone during cartilage formation also resulted in an inhibition, but this was shown to be due to impaired cell proliferation rather than to a direct effect of somatostatin on differentiation. Injection of somatostatin into developing bone tissue after the cartilage stage impaired osteogenesis, assessed by45Ca incorporation and alkaline phosphatase activity. Concurrent injections of insulin and somatostatin obliterated the inhibitory effect of the latter on cell proliferation. Somatostatin can locally regulate the proliferation and differentiation of chondroprogenitor and osteoprogenitor cells in vivo and may directly contribute to the regulation of bone growth by its ability to counteract the stimulatory effect of insulin.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 425-430 |
| Number of pages | 6 |
| Journal | Calcified Tissue International |
| Volume | 33 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1981 |
| Externally published | Yes |
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Keywords
- Bone induction
- Chondrogenesis
- Insulin
ASJC Scopus subject areas
- Endocrinology
- Orthopedics and Sports Medicine
- Endocrinology, Diabetes and Metabolism
Cite this
Somatostatin can locally inhibit proliferation and differentiation of cartilage and bone precursor cells. / Weiss, Roy E.; Reddi, A Hari; Nimni, M. E.
In: Calcified Tissue International, Vol. 33, No. 1, 12.1981, p. 425-430.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Somatostatin can locally inhibit proliferation and differentiation of cartilage and bone precursor cells
AU - Weiss, Roy E.
AU - Reddi, A Hari
AU - Nimni, M. E.
PY - 1981/12
Y1 - 1981/12
N2 - The influence of somatostatin on discrete stages of collagenous-matrix-induced endochondral bone formation has been investigated. Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75% reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. The minimum effective inhibitory dose of somatostatin was 0.25 μg/day. Twice daily local injections of the hormone during cartilage formation also resulted in an inhibition, but this was shown to be due to impaired cell proliferation rather than to a direct effect of somatostatin on differentiation. Injection of somatostatin into developing bone tissue after the cartilage stage impaired osteogenesis, assessed by45Ca incorporation and alkaline phosphatase activity. Concurrent injections of insulin and somatostatin obliterated the inhibitory effect of the latter on cell proliferation. Somatostatin can locally regulate the proliferation and differentiation of chondroprogenitor and osteoprogenitor cells in vivo and may directly contribute to the regulation of bone growth by its ability to counteract the stimulatory effect of insulin.
AB - The influence of somatostatin on discrete stages of collagenous-matrix-induced endochondral bone formation has been investigated. Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75% reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. The minimum effective inhibitory dose of somatostatin was 0.25 μg/day. Twice daily local injections of the hormone during cartilage formation also resulted in an inhibition, but this was shown to be due to impaired cell proliferation rather than to a direct effect of somatostatin on differentiation. Injection of somatostatin into developing bone tissue after the cartilage stage impaired osteogenesis, assessed by45Ca incorporation and alkaline phosphatase activity. Concurrent injections of insulin and somatostatin obliterated the inhibitory effect of the latter on cell proliferation. Somatostatin can locally regulate the proliferation and differentiation of chondroprogenitor and osteoprogenitor cells in vivo and may directly contribute to the regulation of bone growth by its ability to counteract the stimulatory effect of insulin.
KW - Bone induction
KW - Chondrogenesis
KW - Insulin
UR - http://www.scopus.com/inward/record.url?scp=0019811402&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019811402&partnerID=8YFLogxK
U2 - 10.1007/BF02409466
DO - 10.1007/BF02409466
M3 - Article
VL - 33
SP - 425
EP - 430
JO - Calcified Tissue International
JF - Calcified Tissue International
SN - 0171-967X
IS - 1
ER -