Somatostatin can locally inhibit proliferation and differentiation of cartilage and bone precursor cells

Roy E. Weiss, A Hari Reddi, M. E. Nimni

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The influence of somatostatin on discrete stages of collagenous-matrix-induced endochondral bone formation has been investigated. Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75% reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. The minimum effective inhibitory dose of somatostatin was 0.25 μg/day. Twice daily local injections of the hormone during cartilage formation also resulted in an inhibition, but this was shown to be due to impaired cell proliferation rather than to a direct effect of somatostatin on differentiation. Injection of somatostatin into developing bone tissue after the cartilage stage impaired osteogenesis, assessed by45Ca incorporation and alkaline phosphatase activity. Concurrent injections of insulin and somatostatin obliterated the inhibitory effect of the latter on cell proliferation. Somatostatin can locally regulate the proliferation and differentiation of chondroprogenitor and osteoprogenitor cells in vivo and may directly contribute to the regulation of bone growth by its ability to counteract the stimulatory effect of insulin.

Original languageEnglish (US)
Pages (from-to)425-430
Number of pages6
JournalCalcified Tissue International
Volume33
Issue number1
DOIs
StatePublished - Dec 1981
Externally publishedYes

Fingerprint

Somatostatin
Cartilage
Bone and Bones
Injections
Cell Proliferation
Osteogenesis
Insulin
Ornithine Decarboxylase
Bone Development
Thymidine
Alkaline Phosphatase
Hormones

Keywords

  • Bone induction
  • Chondrogenesis
  • Insulin

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Somatostatin can locally inhibit proliferation and differentiation of cartilage and bone precursor cells. / Weiss, Roy E.; Reddi, A Hari; Nimni, M. E.

In: Calcified Tissue International, Vol. 33, No. 1, 12.1981, p. 425-430.

Research output: Contribution to journalArticle

@article{abc979ded3fb401d9dc70212ad5a6fac,
title = "Somatostatin can locally inhibit proliferation and differentiation of cartilage and bone precursor cells",
abstract = "The influence of somatostatin on discrete stages of collagenous-matrix-induced endochondral bone formation has been investigated. Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75{\%} reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. The minimum effective inhibitory dose of somatostatin was 0.25 μg/day. Twice daily local injections of the hormone during cartilage formation also resulted in an inhibition, but this was shown to be due to impaired cell proliferation rather than to a direct effect of somatostatin on differentiation. Injection of somatostatin into developing bone tissue after the cartilage stage impaired osteogenesis, assessed by45Ca incorporation and alkaline phosphatase activity. Concurrent injections of insulin and somatostatin obliterated the inhibitory effect of the latter on cell proliferation. Somatostatin can locally regulate the proliferation and differentiation of chondroprogenitor and osteoprogenitor cells in vivo and may directly contribute to the regulation of bone growth by its ability to counteract the stimulatory effect of insulin.",
keywords = "Bone induction, Chondrogenesis, Insulin",
author = "Weiss, {Roy E.} and Reddi, {A Hari} and Nimni, {M. E.}",
year = "1981",
month = "12",
doi = "10.1007/BF02409466",
language = "English (US)",
volume = "33",
pages = "425--430",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York",
number = "1",

}

TY - JOUR

T1 - Somatostatin can locally inhibit proliferation and differentiation of cartilage and bone precursor cells

AU - Weiss, Roy E.

AU - Reddi, A Hari

AU - Nimni, M. E.

PY - 1981/12

Y1 - 1981/12

N2 - The influence of somatostatin on discrete stages of collagenous-matrix-induced endochondral bone formation has been investigated. Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75% reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. The minimum effective inhibitory dose of somatostatin was 0.25 μg/day. Twice daily local injections of the hormone during cartilage formation also resulted in an inhibition, but this was shown to be due to impaired cell proliferation rather than to a direct effect of somatostatin on differentiation. Injection of somatostatin into developing bone tissue after the cartilage stage impaired osteogenesis, assessed by45Ca incorporation and alkaline phosphatase activity. Concurrent injections of insulin and somatostatin obliterated the inhibitory effect of the latter on cell proliferation. Somatostatin can locally regulate the proliferation and differentiation of chondroprogenitor and osteoprogenitor cells in vivo and may directly contribute to the regulation of bone growth by its ability to counteract the stimulatory effect of insulin.

AB - The influence of somatostatin on discrete stages of collagenous-matrix-induced endochondral bone formation has been investigated. Local injection of somatostatin, i.e., without any measurable systemic effect, resulted in a 75% reduction of cell proliferation as measured by [3H]thymidine incorporation and ornithine decarboxylase activities. The minimum effective inhibitory dose of somatostatin was 0.25 μg/day. Twice daily local injections of the hormone during cartilage formation also resulted in an inhibition, but this was shown to be due to impaired cell proliferation rather than to a direct effect of somatostatin on differentiation. Injection of somatostatin into developing bone tissue after the cartilage stage impaired osteogenesis, assessed by45Ca incorporation and alkaline phosphatase activity. Concurrent injections of insulin and somatostatin obliterated the inhibitory effect of the latter on cell proliferation. Somatostatin can locally regulate the proliferation and differentiation of chondroprogenitor and osteoprogenitor cells in vivo and may directly contribute to the regulation of bone growth by its ability to counteract the stimulatory effect of insulin.

KW - Bone induction

KW - Chondrogenesis

KW - Insulin

UR - http://www.scopus.com/inward/record.url?scp=0019811402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019811402&partnerID=8YFLogxK

U2 - 10.1007/BF02409466

DO - 10.1007/BF02409466

M3 - Article

C2 - 6117356

AN - SCOPUS:0019811402

VL - 33

SP - 425

EP - 430

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 1

ER -