Somatic pairing of homologs in budding yeast: Existence and modulation

Sean M. Burgess, Nancy Kleckner, Beth M. Weiner

Research output: Contribution to journalArticle

89 Scopus citations

Abstract

FISH analysis of well-spread chromosomes reveals that homologs are paired in vegetatively growing budding yeast diploid cells, via multiple interstitial interactions, and independent of recA homologs and mating type heterozygosity. Pairing is present during G1 and G2, and in cells arrested at G1 by mating pheromone, but is disrupted during S phase. Thus, somatic pairing is qualitatively analogous to premeiotic and early meiotic pairing. S-phase pairing disruption occurs by a complex intranuclear program involving regional, nucleus-wide, and temporal determinants. Pairing is also disrupted in two G2-arrest conditions (cdc13ts and nocodazole). Together these findings suggest that cell cycle signals may provoke pairing disruption by modulating underlying chromosome and/or chromatin structure. Whether the cell chooses to disrupt pairing contacts or not (e.g., S phase and G2 arrest, but not G1 arrest or normal G1 or G2), could be dictated by functional considerations involving homolog/sister discrimination.

Original languageEnglish (US)
Pages (from-to)1627-1641
Number of pages15
JournalGenes and Development
Volume13
Issue number12
StatePublished - Jun 15 1999
Externally publishedYes

Keywords

  • Cell cycle
  • Chromosomes
  • Homologs
  • Pairing
  • Yeast

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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    Burgess, S. M., Kleckner, N., & Weiner, B. M. (1999). Somatic pairing of homologs in budding yeast: Existence and modulation. Genes and Development, 13(12), 1627-1641.