Somatic and neuritic spines on tyrosine hydroxylase–immunopositive cells of rat retina

Anna Fasoli, James Dang, Jeffrey S. Johnson, Aaron H. Gouw, Alex Fogli Iseppe, Andrew Ishida

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Dopamine- and tyrosine hydroxylase–immunopositive cells (TH cells) modulate visually driven signals as they flow through retinal photoreceptor, bipolar, and ganglion cells. Previous studies suggested that TH cells release dopamine from varicose axons arborizing in the inner and outer plexiform layers after glutamatergic synapses depolarize TH cell dendrites in the inner plexiform layer and these depolarizations propagate to the varicosities. Although it has been proposed that these excitatory synapses are formed onto appendages resembling dendritic spines, spines have not been found on TH cells of most species examined to date or on TH cell somata that release dopamine when exposed to glutamate receptor agonists. By use of protocols that preserve proximal retinal neuron morphology, we have examined the shape, distribution, and synapse-related immunoreactivity of adult rat TH cells. We report here that TH cell somata, tapering and varicose inner plexiform layer neurites, and varicose outer plexiform layer neurites all bear spines, that some of these spines are immunopositive for glutamate receptor and postsynaptic density proteins (viz., GluR1, GluR4, NR1, PSD-95, and PSD-93), that TH cell somata and tapering neurites are also immunopositive for a γ-aminobutyric acid (GABA) receptor subunit (GABAARα1), and that a synaptic ribbon-specific protein (RIBEYE) is found adjacent to some colocalizations of GluR1 and TH in the inner plexiform layer. These results identify previously undescribed sites at which glutamatergic and GABAergic inputs may stimulate and inhibit dopamine release, especially at somata and along varicose neurites that emerge from these somata and arborize in various levels of the retina. J. Comp. Neurol. 525:1707–1730, 2017.

Original languageEnglish (US)
Pages (from-to)1707-1730
Number of pages24
JournalJournal of Comparative Neurology
Volume525
Issue number7
DOIs
StatePublished - May 1 2017

Fingerprint

Tyrosine
Retina
Spine
Carisoprodol
Neurites
Dopamine
Synapses
Glutamate Receptors
Retinal Bipolar Cells
Aminobutyrates
Retinal Neurons
Excitatory Amino Acid Agonists
Dendritic Spines
Vertebrate Photoreceptor Cells
GABA Receptors
Retinal Ganglion Cells
Dendrites
Axons
Proteins

Keywords

  • axons
  • dendrites
  • dopamine
  • interplexiform cells
  • retina
  • RRID: AB_11001825 (antibody_PSD-93)
  • RRID: AB_2201528 (antibody_TH MS)
  • RRID: AB_2213602 (antibody_GluR1)
  • RRID: AB_2307331 (antibody_PSD-95)
  • RRID: AB_2314955 (antibody_NMDAR1)
  • RRID: AB_2315778 (antibody_DKαSHcy3)
  • RRID: AB_2338694 (antibody_GTαIgG1MSCy3)
  • RRID: AB_2338854 (antibody_GTαIgG1MSaf488)
  • RRID: AB_2338917 (antibody_GTαIgG2aMSaf647)
  • RRID: AB_2340863 (antibody_DKαMSaf647)
  • RRID: AB_2576217 (antibody_GTαRBaf488)
  • RRID: AB_310272 (antibody_GABAA_α1)
  • RRID: AB_399431 (antibody_RIBEYE)
  • RRID: AB_90711 (antibody_GluR4)
  • RRID: AB_90755 (antibody_TH_SH)
  • RRID: NLX_143660 (database_JCNantibody)
  • RRID: RGD_1566443 (organism_Lrat)
  • RRID: RGD_60991 (organism_LErat)
  • RRID: SCR_001622 (MatLab)
  • RRID: SCR_001905 (R Project for Statistical Computing)
  • RRID: SCR_002285 (Fiji)
  • RRID: SCR_007370 (Imaris)
  • RRID: SCR_014237 (Huygens)
  • spines

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fasoli, A., Dang, J., Johnson, J. S., Gouw, A. H., Fogli Iseppe, A., & Ishida, A. (2017). Somatic and neuritic spines on tyrosine hydroxylase–immunopositive cells of rat retina. Journal of Comparative Neurology, 525(7), 1707-1730. https://doi.org/10.1002/cne.24166

Somatic and neuritic spines on tyrosine hydroxylase–immunopositive cells of rat retina. / Fasoli, Anna; Dang, James; Johnson, Jeffrey S.; Gouw, Aaron H.; Fogli Iseppe, Alex; Ishida, Andrew.

In: Journal of Comparative Neurology, Vol. 525, No. 7, 01.05.2017, p. 1707-1730.

Research output: Contribution to journalArticle

Fasoli, A, Dang, J, Johnson, JS, Gouw, AH, Fogli Iseppe, A & Ishida, A 2017, 'Somatic and neuritic spines on tyrosine hydroxylase–immunopositive cells of rat retina', Journal of Comparative Neurology, vol. 525, no. 7, pp. 1707-1730. https://doi.org/10.1002/cne.24166
Fasoli, Anna ; Dang, James ; Johnson, Jeffrey S. ; Gouw, Aaron H. ; Fogli Iseppe, Alex ; Ishida, Andrew. / Somatic and neuritic spines on tyrosine hydroxylase–immunopositive cells of rat retina. In: Journal of Comparative Neurology. 2017 ; Vol. 525, No. 7. pp. 1707-1730.
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AU - Dang, James

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AU - Ishida, Andrew

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N2 - Dopamine- and tyrosine hydroxylase–immunopositive cells (TH cells) modulate visually driven signals as they flow through retinal photoreceptor, bipolar, and ganglion cells. Previous studies suggested that TH cells release dopamine from varicose axons arborizing in the inner and outer plexiform layers after glutamatergic synapses depolarize TH cell dendrites in the inner plexiform layer and these depolarizations propagate to the varicosities. Although it has been proposed that these excitatory synapses are formed onto appendages resembling dendritic spines, spines have not been found on TH cells of most species examined to date or on TH cell somata that release dopamine when exposed to glutamate receptor agonists. By use of protocols that preserve proximal retinal neuron morphology, we have examined the shape, distribution, and synapse-related immunoreactivity of adult rat TH cells. We report here that TH cell somata, tapering and varicose inner plexiform layer neurites, and varicose outer plexiform layer neurites all bear spines, that some of these spines are immunopositive for glutamate receptor and postsynaptic density proteins (viz., GluR1, GluR4, NR1, PSD-95, and PSD-93), that TH cell somata and tapering neurites are also immunopositive for a γ-aminobutyric acid (GABA) receptor subunit (GABAARα1), and that a synaptic ribbon-specific protein (RIBEYE) is found adjacent to some colocalizations of GluR1 and TH in the inner plexiform layer. These results identify previously undescribed sites at which glutamatergic and GABAergic inputs may stimulate and inhibit dopamine release, especially at somata and along varicose neurites that emerge from these somata and arborize in various levels of the retina. J. Comp. Neurol. 525:1707–1730, 2017.

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KW - RRID: AB_11001825 (antibody_PSD-93)

KW - RRID: AB_2201528 (antibody_TH MS)

KW - RRID: AB_2213602 (antibody_GluR1)

KW - RRID: AB_2307331 (antibody_PSD-95)

KW - RRID: AB_2314955 (antibody_NMDAR1)

KW - RRID: AB_2315778 (antibody_DKαSHcy3)

KW - RRID: AB_2338694 (antibody_GTαIgG1MSCy3)

KW - RRID: AB_2338854 (antibody_GTαIgG1MSaf488)

KW - RRID: AB_2338917 (antibody_GTαIgG2aMSaf647)

KW - RRID: AB_2340863 (antibody_DKαMSaf647)

KW - RRID: AB_2576217 (antibody_GTαRBaf488)

KW - RRID: AB_310272 (antibody_GABAA_α1)

KW - RRID: AB_399431 (antibody_RIBEYE)

KW - RRID: AB_90711 (antibody_GluR4)

KW - RRID: AB_90755 (antibody_TH_SH)

KW - RRID: NLX_143660 (database_JCNantibody)

KW - RRID: RGD_1566443 (organism_Lrat)

KW - RRID: RGD_60991 (organism_LErat)

KW - RRID: SCR_001622 (MatLab)

KW - RRID: SCR_001905 (R Project for Statistical Computing)

KW - RRID: SCR_002285 (Fiji)

KW - RRID: SCR_007370 (Imaris)

KW - RRID: SCR_014237 (Huygens)

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