Solution structure of heavy meromyosin by small-angle scattering

Samantha P. Harris, William T. Heller, Marion L. Greaser, Richard L. Moss, Jill Trewhella

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Elucidation of x-ray crystal structures for the S1 subfragment of myosin afforded atomic resolution of the nucleotide and actin binding sites of the enzyme. The structures have led to more detailed hypotheses regarding the mechanisms by which force generation is coupled to ATP hydrolysis. However, the three-dimensional structure of double-headed myosin consisting of two S1 subfragments has not yet been solved. Therefore, to investigate the overall shape and relative orientations of the two heads of myosin, we performed small-angle x-ray and neutron scattering measurements of heavy meromyosin containing all three light chains (LC1-3) in solution. The resulting small-angle scattering intensity profiles were best fit by models of the heavy meromyosin head-tail junction in which the angular separation between heads was less than 180 degrees. The S1 heads of the best fit models are not related by an axis of symmetry, and one of the two S1 heads is bent back along the rod. These results provide new information on the structure of the head-tail junction of myosin and indicate that combining scattering measurements with high resolution structural modeling is a feasible approach for investigating myosin head-head interactions in solution.

Original languageEnglish (US)
Pages (from-to)6034-6040
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number8
DOIs
StatePublished - Feb 21 2003
Externally publishedYes

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Small Angle Scattering
Myosin Subfragments
Myosins
Head
Scattering
X rays
X-Rays
Neutron scattering
Neutrons
Actins
Hydrolysis
Nucleotides
Adenosine Triphosphate
Crystal structure
Binding Sites
Enzymes
Light

ASJC Scopus subject areas

  • Biochemistry

Cite this

Harris, S. P., Heller, W. T., Greaser, M. L., Moss, R. L., & Trewhella, J. (2003). Solution structure of heavy meromyosin by small-angle scattering. Journal of Biological Chemistry, 278(8), 6034-6040. https://doi.org/10.1074/jbc.M210558200

Solution structure of heavy meromyosin by small-angle scattering. / Harris, Samantha P.; Heller, William T.; Greaser, Marion L.; Moss, Richard L.; Trewhella, Jill.

In: Journal of Biological Chemistry, Vol. 278, No. 8, 21.02.2003, p. 6034-6040.

Research output: Contribution to journalArticle

Harris, SP, Heller, WT, Greaser, ML, Moss, RL & Trewhella, J 2003, 'Solution structure of heavy meromyosin by small-angle scattering', Journal of Biological Chemistry, vol. 278, no. 8, pp. 6034-6040. https://doi.org/10.1074/jbc.M210558200
Harris SP, Heller WT, Greaser ML, Moss RL, Trewhella J. Solution structure of heavy meromyosin by small-angle scattering. Journal of Biological Chemistry. 2003 Feb 21;278(8):6034-6040. https://doi.org/10.1074/jbc.M210558200
Harris, Samantha P. ; Heller, William T. ; Greaser, Marion L. ; Moss, Richard L. ; Trewhella, Jill. / Solution structure of heavy meromyosin by small-angle scattering. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 8. pp. 6034-6040.
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