Soluble epoxide hydrolase pharmacological inhibition decreases alveolar bone loss by modulating host inflammatory response, rank-related signaling, endoplasmic reticulum stress, and apoptosis

Carlos Antonio Trindade-Da-Silva, Ahmed Bettaieb, Marcelo Henrique Napimoga, Kin Sing Stephen Lee, Bora Inceoglu, Carlos Ueira-Vieira, Donald Bruun, Sumanta Kumar Goswami, Fawaz Haj, Bruce D. Hammock

Research output: Contribution to journalArticle

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Abstract

Epoxyeicosatrienoic acids (EETs), metabolites of arachidonic acid derived from the cytochrome P450 enzymes, are mainly metabolized by soluble epoxide hydrolase (sEH) to their corresponding diols. EETs but not their diols, have anti-inflammatory properties, and inhibition of sEH might provide protective effects against inflammatory bone loss. Thus, in the present study, we tested the selective sEH inhibitor, 1-trifluoromethoxyphenyl-3-(1-propionylpiperidin-4-yl) urea (TPPU), in a mouse model of periodontitis induced by infection with Aggregatibacter actinomycetemcomitans. Oral treatment of wild-type mice with TPPU and sEH knockout (KO) animals showed reduced bone loss induced by A. actinomycetemcomitans. This was associated with decreased expression of key osteoclastogenic molecules, receptor activator of nuclear factor-kB/RANK ligand/osteoprotegerin, and the chemokine monocyte chemotactic protein 1 in the gingival tissue without affecting bacterial counts. In addition, downstream kinases p38 and c-Jun N-terminal kinase known to be activated in response to inflammatory signals were abrogated after TPPU treatment or in sEH KO mice. Moreover, endoplasmic reticulum stress was elevated in periodontal disease but was abrogated after TPPU treatment and in sEH knockout mice. Together, these results demonstrated that sEH pharmacological inhibition may be of therapeutic value in periodontitis.

Original languageEnglish (US)
Pages (from-to)408-416
Number of pages9
JournalJournal of Pharmacology and Experimental Therapeutics
Volume361
Issue number3
DOIs
StatePublished - Jun 1 2017

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Alveolar Bone Loss
Epoxide Hydrolases
Endoplasmic Reticulum Stress
Pharmacology
Apoptosis
Aggregatibacter actinomycetemcomitans
Periodontitis
Knockout Mice
Cytochrome P-450 Enzyme System
RANK Ligand
Osteoprotegerin
Bone and Bones
Bacterial Load
JNK Mitogen-Activated Protein Kinases
Chemokine CCL2
Periodontal Diseases
Cytoplasmic and Nuclear Receptors
Chemokines
Arachidonic Acid
Urea

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

Cite this

Soluble epoxide hydrolase pharmacological inhibition decreases alveolar bone loss by modulating host inflammatory response, rank-related signaling, endoplasmic reticulum stress, and apoptosis. / Trindade-Da-Silva, Carlos Antonio; Bettaieb, Ahmed; Napimoga, Marcelo Henrique; Lee, Kin Sing Stephen; Inceoglu, Bora; Ueira-Vieira, Carlos; Bruun, Donald; Goswami, Sumanta Kumar; Haj, Fawaz; Hammock, Bruce D.

In: Journal of Pharmacology and Experimental Therapeutics, Vol. 361, No. 3, 01.06.2017, p. 408-416.

Research output: Contribution to journalArticle

Trindade-Da-Silva, Carlos Antonio ; Bettaieb, Ahmed ; Napimoga, Marcelo Henrique ; Lee, Kin Sing Stephen ; Inceoglu, Bora ; Ueira-Vieira, Carlos ; Bruun, Donald ; Goswami, Sumanta Kumar ; Haj, Fawaz ; Hammock, Bruce D. / Soluble epoxide hydrolase pharmacological inhibition decreases alveolar bone loss by modulating host inflammatory response, rank-related signaling, endoplasmic reticulum stress, and apoptosis. In: Journal of Pharmacology and Experimental Therapeutics. 2017 ; Vol. 361, No. 3. pp. 408-416.
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