Soluble epoxide hydrolase deficiency inhibits dextran sulfate sodium-induced colitis and carcinogenesis in mice

Wanying Zhang, Haonan Li, Hua Dong, Jie Liao, Bruce D. Hammock, Guang Yu Yang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Soluble epoxide hydrolase (sEH) hydrolyses/inactivates anti-inflammatory epoxyeicosatrienoic acids (EETs) to their corresponding diols, and targeting sEH leads to strong anti-inflammatory effects. In the present study, using a tissue microarray and immunohistochemical approach, a significant increase of sEH expression was identified in ulcerative colitis (UC)-associated dysplasia and adenocarcinoma. The effects of deficiency in the sEH gene were determined on dextran sulfate sodium (DSS) colitis-induced carcinogenesis. The effects of EETs on lipopolysaccharide (LPS)-activated macrophages were analyzed in vitro. With extensive histopathological and immunohistochemical analyses, compared to wild-type mice, sEH-/- mice exhibited a significant decrease in tumor incidence (13/20 vs. 6/19, p<0.05) and a markedly reduced average tumor size (59.62±20.91 mm3 vs. 22.42±11.22 mm3), and a significant number of pre-cancerous dysplasia (3±1.18 vs. 2±0.83, p<0.01). The inflammatory activity, as measured by the extent/proportion of erosion/ulceration/dense lymphoplasmacytosis (called active colitis index) in the colon, was significantly lower in sEH-/- mice (44.7%±24.9% vs. 20.2%±16.2%, p<0.01). The quantitative polymerase chain reaction (qPCR) assays demonstrated significantly low levels of cytokines/chemokines including monocyte chemoattractant protein (MCP-1), inducible nitric oxide synthase (iNOS), vasopressin-activated calcium-mobilizing (VCAM-1), interleukin-1 beta (IL-1β) and tumor necrosis factor-alpha (TNF-α). In vitro, LPS-activated macrophages treated with 14,15-EET showed a significant reduction of LPS-triggered IL-1β and TNF-α expression. Eicosanoic acid metabolic profiling revealed a significant increase of the ratios of EETs/ dihydroeicosatrienoic acids (DHETs) and epoxyoctadecennoic acid/ dihydroxyoctadecenoic acid (EpOMEs/DiHOMEs). These results indicate that sEH plays an important role in the development of colitis and in inducing carcinogenesis.

Original languageEnglish (US)
Pages (from-to)5261-5272
Number of pages12
JournalAnticancer Research
Volume33
Issue number12
StatePublished - Dec 2013

Fingerprint

Epoxide Hydrolases
Dextran Sulfate
Colitis
Carcinogenesis
Lipopolysaccharides
Acids
Interleukin-1beta
Eicosanoic Acids
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Macrophages
Vascular Cell Adhesion Molecule-1
Chemokine CCL2
Nitric Oxide Synthase Type II
Vasopressins
Ulcerative Colitis
Chemokines
Neoplasms
Colon
Adenocarcinoma

Keywords

  • Carcinogenesis
  • Colitis
  • Dextran sulfate sodium
  • Mice
  • Soluble epoxide hydrolase

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Zhang, W., Li, H., Dong, H., Liao, J., Hammock, B. D., & Yang, G. Y. (2013). Soluble epoxide hydrolase deficiency inhibits dextran sulfate sodium-induced colitis and carcinogenesis in mice. Anticancer Research, 33(12), 5261-5272.

Soluble epoxide hydrolase deficiency inhibits dextran sulfate sodium-induced colitis and carcinogenesis in mice. / Zhang, Wanying; Li, Haonan; Dong, Hua; Liao, Jie; Hammock, Bruce D.; Yang, Guang Yu.

In: Anticancer Research, Vol. 33, No. 12, 12.2013, p. 5261-5272.

Research output: Contribution to journalArticle

Zhang, W, Li, H, Dong, H, Liao, J, Hammock, BD & Yang, GY 2013, 'Soluble epoxide hydrolase deficiency inhibits dextran sulfate sodium-induced colitis and carcinogenesis in mice', Anticancer Research, vol. 33, no. 12, pp. 5261-5272.
Zhang, Wanying ; Li, Haonan ; Dong, Hua ; Liao, Jie ; Hammock, Bruce D. ; Yang, Guang Yu. / Soluble epoxide hydrolase deficiency inhibits dextran sulfate sodium-induced colitis and carcinogenesis in mice. In: Anticancer Research. 2013 ; Vol. 33, No. 12. pp. 5261-5272.
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