Solitary horizontal cells in culture-II. A new tool for examining effects of photoreceptor neurotransmitter candidates

Andrew Ishida, A. K. Aneko, M. Tachibana

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Membrane potentials of solitary horizontal cells dissociated from goldfish retinas were intracellularly measured while applying various acidic amino acids. l-glutamate and kainic acid depolarized solitary horizontal cells at micromolar doses. Neither l- nor d-aspartate produced any change in solitary horizontal cell resting potentials. Low-amplitude responses to l-glutamate showed no sign of desensitization. A steady, plateau-like, dose-dependent component of solitary horizontal cell responses to either l-glutamate or kainic acid, though obscured during its rising phase by action potentials, was always recorded during maintained agonist applications. Responses to either lglutamate or kainic acid reversed in polarity at membrane potentials between 0 and t-20 mV. Responses to l-glutamate collapsed reversibly when extracellular sodium ions were replaced by choline ions. Responses to either l-glutamate or kainic acid were antagonized by relatively high doses of d-aspartate. These results demonstrate that retinal horizontal cell chemosensitivity to acidic amino acids persists after dissociation, and in several respects resembles that found in several other preparations, including retinas in situ.

Original languageEnglish (US)
Pages (from-to)1217-1220
Number of pages4
JournalVision Research
Volume23
Issue number11
DOIs
StatePublished - 1983
Externally publishedYes

Keywords

  • Goldfish
  • Horizontal cells l-glutamate
  • Neurotransmitter
  • Photoreceptor
  • Retina

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

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