Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR

Prerak T. Desai; Marie K. Walsh; Bart C Weimer

doi:10.1128/AEM.02601-07

Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR

Prerak T. Desai, Marie K. Walsh, Bart C Weimer

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of ∼4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

Original language	English (US)
Pages (from-to)	2254-2258
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	74
Issue number	7
DOIs	https://doi.org/10.1128/AEM.02601-07
State	Published - Apr 2008
Externally published	Yes

ASJC Scopus subject areas

Environmental Science(all)
Biotechnology
Microbiology

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abstract = "We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of ∼4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.",

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