Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR

Prerak T. Desai, Marie K. Walsh, Bart C Weimer

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of ∼4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.

Original languageEnglish (US)
Pages (from-to)2254-2258
Number of pages5
JournalApplied and Environmental Microbiology
Volume74
Issue number7
DOIs
StatePublished - Apr 2008
Externally publishedYes

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

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