Abstract
We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of ∼4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2254-2258 |
| Number of pages | 5 |
| Journal | Applied and Environmental Microbiology |
| Volume | 74 |
| Issue number | 7 |
| DOIs | |
| State | Published - Apr 2008 |
| Externally published | Yes |
ASJC Scopus subject areas
- Environmental Science(all)
- Biotechnology
- Microbiology
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Cite this
Desai, P. T., Walsh, M. K., & Weimer, B. C. (2008). Solid-phase capture of pathogenic bacteria by using gangliosides and detection with real-time PCR. Applied and Environmental Microbiology, 74(7), 2254-2258. https://doi.org/10.1128/AEM.02601-07