Abstract
We developed a method for concentrating pathogens from samples without enrichment. Immobilized gangliosides concentrated bacteria for detection with real-time PCR. A sensitivity of ∼4 CFU/ml (3 h) in samples without competing microflora was achieved. Samples with competing microflora had a sensitivity of 40,000 CFU/ml. The variance was less than one cycle.
Original language | English (US) |
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Pages (from-to) | 2254-2258 |
Number of pages | 5 |
Journal | Applied and Environmental Microbiology |
Volume | 74 |
Issue number | 7 |
DOIs | |
State | Published - Apr 2008 |
Externally published | Yes |
ASJC Scopus subject areas
- Environmental Science(all)
- Biotechnology
- Microbiology