Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart

Victor D. Schepkin, I. Oojin Choy, Thomas F. Budinger, Derek Y. Obayashi, Scott E. Taylor, William M. DeCampli, Sundar C. Amartur, J Nilas Young

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


The feasibility of monitoring intracellular sodium changes using Na triple quantum filtered NMR without a chemical shift reagent (SR) was investigated in an isolated rat heart during a variety of interventions for Na, loading. Perfusion with 1 mM ouabain or without K+ present in the perfusate for 30 min produced a rise of the Na TQF signal with a plateau of ~190% and ~228% relative to the preintervention level, respectively. Stop- flow ischemia for 30 min resulted in a TQF signal growth of ~147%. The maximal Na TQF signal increase of 460% was achieved by perfusion without K+/Ca2+, corresponding to an elimination of the Na transmembrane gradient. The observed values of Na NMR TQF growth in the physiological and pathological ranges are in agreement with reported data by other methods and have a linear correlation with intracellular sodium content as determined in this study by Co-EDTA method and by sucrose-histidine washout of the extracellular space. Our data indicate that the increase in Na TQF NMR signal is determined by the growth of Na(i), and the extracellular Na contribution to the total TQF signal is unchanged at ~64%. In conclusion, Na TQF NMR without using SR offers a unique and noninvasive opportunity to monitor alterations of intracellular sodium. It may provide valuable insights for developing cardioprotentive strategies and for observing the effects of pharmaceutical treatments on sodium homeostasis.

Original languageEnglish (US)
Pages (from-to)557-563
Number of pages7
JournalMagnetic Resonance in Medicine
Issue number4
StatePublished - Apr 1998
Externally publishedYes


  • Calcium paradox
  • Intracellular sodium
  • Ischemia
  • Triple quantum filter

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology


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