Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart

Victor D. Schepkin, I. Oojin Choy, Thomas F. Budinger, Derek Y. Obayashi, Scott E. Taylor, William M. DeCampli, Sundar C. Amartur, J Nilas Young

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The feasibility of monitoring intracellular sodium changes using Na triple quantum filtered NMR without a chemical shift reagent (SR) was investigated in an isolated rat heart during a variety of interventions for Na, loading. Perfusion with 1 mM ouabain or without K+ present in the perfusate for 30 min produced a rise of the Na TQF signal with a plateau of ~190% and ~228% relative to the preintervention level, respectively. Stop- flow ischemia for 30 min resulted in a TQF signal growth of ~147%. The maximal Na TQF signal increase of 460% was achieved by perfusion without K+/Ca2+, corresponding to an elimination of the Na transmembrane gradient. The observed values of Na NMR TQF growth in the physiological and pathological ranges are in agreement with reported data by other methods and have a linear correlation with intracellular sodium content as determined in this study by Co-EDTA method and by sucrose-histidine washout of the extracellular space. Our data indicate that the increase in Na TQF NMR signal is determined by the growth of Na(i), and the extracellular Na contribution to the total TQF signal is unchanged at ~64%. In conclusion, Na TQF NMR without using SR offers a unique and noninvasive opportunity to monitor alterations of intracellular sodium. It may provide valuable insights for developing cardioprotentive strategies and for observing the effects of pharmaceutical treatments on sodium homeostasis.

Original languageEnglish (US)
Pages (from-to)557-563
Number of pages7
JournalMagnetic Resonance in Medicine
Volume39
Issue number4
DOIs
StatePublished - Apr 1998
Externally publishedYes

Fingerprint

Sodium
Growth
Perfusion
Extracellular Space
Ouabain
Histidine
Edetic Acid
Sucrose
Homeostasis
Ischemia
crystalloid solutions
Pharmaceutical Preparations

Keywords

  • Calcium paradox
  • Intracellular sodium
  • Ischemia
  • Triple quantum filter

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Schepkin, V. D., Choy, I. O., Budinger, T. F., Obayashi, D. Y., Taylor, S. E., DeCampli, W. M., ... Young, J. N. (1998). Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart. Magnetic Resonance in Medicine, 39(4), 557-563. https://doi.org/10.1002/mrm.1910390408

Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart. / Schepkin, Victor D.; Choy, I. Oojin; Budinger, Thomas F.; Obayashi, Derek Y.; Taylor, Scott E.; DeCampli, William M.; Amartur, Sundar C.; Young, J Nilas.

In: Magnetic Resonance in Medicine, Vol. 39, No. 4, 04.1998, p. 557-563.

Research output: Contribution to journalArticle

Schepkin, VD, Choy, IO, Budinger, TF, Obayashi, DY, Taylor, SE, DeCampli, WM, Amartur, SC & Young, JN 1998, 'Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart', Magnetic Resonance in Medicine, vol. 39, no. 4, pp. 557-563. https://doi.org/10.1002/mrm.1910390408
Schepkin VD, Choy IO, Budinger TF, Obayashi DY, Taylor SE, DeCampli WM et al. Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart. Magnetic Resonance in Medicine. 1998 Apr;39(4):557-563. https://doi.org/10.1002/mrm.1910390408
Schepkin, Victor D. ; Choy, I. Oojin ; Budinger, Thomas F. ; Obayashi, Derek Y. ; Taylor, Scott E. ; DeCampli, William M. ; Amartur, Sundar C. ; Young, J Nilas. / Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart. In: Magnetic Resonance in Medicine. 1998 ; Vol. 39, No. 4. pp. 557-563.
@article{c20a635018c9468a960cc77a1360edc4,
title = "Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart",
abstract = "The feasibility of monitoring intracellular sodium changes using Na triple quantum filtered NMR without a chemical shift reagent (SR) was investigated in an isolated rat heart during a variety of interventions for Na, loading. Perfusion with 1 mM ouabain or without K+ present in the perfusate for 30 min produced a rise of the Na TQF signal with a plateau of ~190{\%} and ~228{\%} relative to the preintervention level, respectively. Stop- flow ischemia for 30 min resulted in a TQF signal growth of ~147{\%}. The maximal Na TQF signal increase of 460{\%} was achieved by perfusion without K+/Ca2+, corresponding to an elimination of the Na transmembrane gradient. The observed values of Na NMR TQF growth in the physiological and pathological ranges are in agreement with reported data by other methods and have a linear correlation with intracellular sodium content as determined in this study by Co-EDTA method and by sucrose-histidine washout of the extracellular space. Our data indicate that the increase in Na TQF NMR signal is determined by the growth of Na(i), and the extracellular Na contribution to the total TQF signal is unchanged at ~64{\%}. In conclusion, Na TQF NMR without using SR offers a unique and noninvasive opportunity to monitor alterations of intracellular sodium. It may provide valuable insights for developing cardioprotentive strategies and for observing the effects of pharmaceutical treatments on sodium homeostasis.",
keywords = "Calcium paradox, Intracellular sodium, Ischemia, Triple quantum filter",
author = "Schepkin, {Victor D.} and Choy, {I. Oojin} and Budinger, {Thomas F.} and Obayashi, {Derek Y.} and Taylor, {Scott E.} and DeCampli, {William M.} and Amartur, {Sundar C.} and Young, {J Nilas}",
year = "1998",
month = "4",
doi = "10.1002/mrm.1910390408",
language = "English (US)",
volume = "39",
pages = "557--563",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Sodium TQF NMR and intracellular sodium in isolated crystalloid perfused rat heart

AU - Schepkin, Victor D.

AU - Choy, I. Oojin

AU - Budinger, Thomas F.

AU - Obayashi, Derek Y.

AU - Taylor, Scott E.

AU - DeCampli, William M.

AU - Amartur, Sundar C.

AU - Young, J Nilas

PY - 1998/4

Y1 - 1998/4

N2 - The feasibility of monitoring intracellular sodium changes using Na triple quantum filtered NMR without a chemical shift reagent (SR) was investigated in an isolated rat heart during a variety of interventions for Na, loading. Perfusion with 1 mM ouabain or without K+ present in the perfusate for 30 min produced a rise of the Na TQF signal with a plateau of ~190% and ~228% relative to the preintervention level, respectively. Stop- flow ischemia for 30 min resulted in a TQF signal growth of ~147%. The maximal Na TQF signal increase of 460% was achieved by perfusion without K+/Ca2+, corresponding to an elimination of the Na transmembrane gradient. The observed values of Na NMR TQF growth in the physiological and pathological ranges are in agreement with reported data by other methods and have a linear correlation with intracellular sodium content as determined in this study by Co-EDTA method and by sucrose-histidine washout of the extracellular space. Our data indicate that the increase in Na TQF NMR signal is determined by the growth of Na(i), and the extracellular Na contribution to the total TQF signal is unchanged at ~64%. In conclusion, Na TQF NMR without using SR offers a unique and noninvasive opportunity to monitor alterations of intracellular sodium. It may provide valuable insights for developing cardioprotentive strategies and for observing the effects of pharmaceutical treatments on sodium homeostasis.

AB - The feasibility of monitoring intracellular sodium changes using Na triple quantum filtered NMR without a chemical shift reagent (SR) was investigated in an isolated rat heart during a variety of interventions for Na, loading. Perfusion with 1 mM ouabain or without K+ present in the perfusate for 30 min produced a rise of the Na TQF signal with a plateau of ~190% and ~228% relative to the preintervention level, respectively. Stop- flow ischemia for 30 min resulted in a TQF signal growth of ~147%. The maximal Na TQF signal increase of 460% was achieved by perfusion without K+/Ca2+, corresponding to an elimination of the Na transmembrane gradient. The observed values of Na NMR TQF growth in the physiological and pathological ranges are in agreement with reported data by other methods and have a linear correlation with intracellular sodium content as determined in this study by Co-EDTA method and by sucrose-histidine washout of the extracellular space. Our data indicate that the increase in Na TQF NMR signal is determined by the growth of Na(i), and the extracellular Na contribution to the total TQF signal is unchanged at ~64%. In conclusion, Na TQF NMR without using SR offers a unique and noninvasive opportunity to monitor alterations of intracellular sodium. It may provide valuable insights for developing cardioprotentive strategies and for observing the effects of pharmaceutical treatments on sodium homeostasis.

KW - Calcium paradox

KW - Intracellular sodium

KW - Ischemia

KW - Triple quantum filter

UR - http://www.scopus.com/inward/record.url?scp=0031922579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031922579&partnerID=8YFLogxK

U2 - 10.1002/mrm.1910390408

DO - 10.1002/mrm.1910390408

M3 - Article

C2 - 9543417

AN - SCOPUS:0031922579

VL - 39

SP - 557

EP - 563

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 4

ER -