Sodium butyrate upregulates Kupffer cell PGE2 production and modulates immune function

Richard V Perez, Frazier Stevenson, Jeremy Johnson, Murray Morgan, Kent L Erickson, Neil Hubbard, Larry Morand, Steven Rudich, Steven Katznelson, J. Bruce German

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


The immunosuppressive effect of portal venous blood transfusions in organ transplantation has been well established and may be mediated by increased Kupffer cell production of the immunosuppressive arachidonic acid metabolite prostaglandin E2 (PGE2). In this study, butyrate, a short-chain fatty acid known to enhance gene transcription, is hypothesized to enhance Kupffer cell PGE2 production by altering cyclooxygenase or phospholipase A2 (PLA2) activity, thus augmenting the immunosuppressive effect of portal venous transfusion. Lewis rats were given a portal venous transfusion of Wistar-Firth blood or saline 1 h prior to Kupffer cell harvest. The in vitro effects of butyrate on Kupffer cell PGE2 production, cyclooxygenase, and PLA2 activity were assessed. Kupffer cell tumor necrosis factor-alpha (TNFα) production was also assessed due to its sensitivity to PGE2 and its proinflamatory effects. Kupffer cells from portally transfused animals produced significantly more PGE2 than saline-transfused controls. Addition of butyrate to the culture medium further increased PGE2 production by as much as sevenfold in Kupffer cells of portally transfused animals. Other short-chain fatty acids, propionate and hexanoate, did not increase PGE2 production. Butyrate added to Kupffer cells from transfused animals slightly upregulated inducible cyclooxygenase (COX-2) mRNA levels as measured by both Northern blot and reverse-transcriptase polymerase chain reaction and increased PLA2 activity fivefold as measured by Western blot. Kupffer cell immune function was also affected by in vitro butyrate treatment with a significant decrease in the production of TNFα. Thus, butyrate may be a useful immunoregulatory agent in organ transplantation protocols which seek to enhance transcription of immunosuppressive molecules.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalJournal of Surgical Research
Issue number1
StatePublished - Jul 15 1998

ASJC Scopus subject areas

  • Surgery


Dive into the research topics of 'Sodium butyrate upregulates Kupffer cell PGE<sub>2</sub> production and modulates immune function'. Together they form a unique fingerprint.

Cite this