Abstract
Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.
| Original language | Undefined/Unknown |
|---|---|
| Pages (from-to) | 207-214 |
| Number of pages | 8 |
| Journal | Neirofiziologiia = Neurophysiology |
| Volume | 18 |
| Issue number | 2 |
| State | Published - 1986 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Physiology
Cite this
Issledovanie natrievykh i kal'tsievykh kanalov somaticheskoǐ membrany kletok neǐroblastomy v protsesse ikh iskusstvenno vyzvannoǐ differentsirovki. / Veselovskiǐ, N. S.; Fomina, Alla F.
In: Neirofiziologiia = Neurophysiology, Vol. 18, No. 2, 1986, p. 207-214.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Issledovanie natrievykh i kal'tsievykh kanalov somaticheskoǐ membrany kletok neǐroblastomy v protsesse ikh iskusstvenno vyzvannoǐ differentsirovki.
AU - Veselovskiǐ, N. S.
AU - Fomina, Alla F
PY - 1986
Y1 - 1986
N2 - Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.
AB - Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.
UR - http://www.scopus.com/inward/record.url?scp=0022566747&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022566747&partnerID=8YFLogxK
M3 - Article
C2 - 2423892
AN - SCOPUS:0022566747
VL - 18
SP - 207
EP - 214
JO - Neirofiziologiya
JF - Neirofiziologiya
SN - 0028-2561
IS - 2
ER -