Issledovanie natrievykh i kal'tsievykh kanalov somaticheskoǐ membrany kletok neǐroblastomy v protsesse ikh iskusstvenno vyzvannoǐ differentsirovki.

Translated title of the contribution: Sodium and calcium channels of the somatic membrane of neuroblastoma cells during artificially induced differentiation

N. S. Veselovskiǐ, Alla F Fomina

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.

Original languageUndefined/Unknown
Pages (from-to)207-214
Number of pages8
JournalNeirofiziologiia = Neurophysiology
Volume18
Issue number2
StatePublished - 1986
Externally publishedYes

Fingerprint

Sodium Channels
Calcium Channels
Neuroblastoma
Cell Membrane
Suspensions
Culture Media
Patch-Clamp Techniques
Dialysis
Sodium
Calcium

ASJC Scopus subject areas

  • Physiology

Cite this

@article{676d7db6a9f9413783a5eb9d1472a29e,
title = "Issledovanie natrievykh i kal'tsievykh kanalov somaticheskoǐ membrany kletok neǐroblastomy v protsesse ikh iskusstvenno vyzvannoǐ differentsirovki.",
abstract = "Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.",
author = "Veselovskiǐ, {N. S.} and Fomina, {Alla F}",
year = "1986",
language = "Undefined/Unknown",
volume = "18",
pages = "207--214",
journal = "Neirofiziologiya",
issn = "0028-2561",
number = "2",

}

TY - JOUR

T1 - Issledovanie natrievykh i kal'tsievykh kanalov somaticheskoǐ membrany kletok neǐroblastomy v protsesse ikh iskusstvenno vyzvannoǐ differentsirovki.

AU - Veselovskiǐ, N. S.

AU - Fomina, Alla F

PY - 1986

Y1 - 1986

N2 - Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.

AB - Sodium and calcium channels passing inward currents were studied by intracellular dialysis technique and voltage clamp in the somatic membrane of neuroblastoma cells during their morphological differentiation induced by increasing pH of the culture medium up to 8.0-8.2. Kinetic and voltage-dependent properties of sodium and calcium channels of differentiated cells and cells grown in the suspension culture were identical. Densities of sodium currents in the somatic membrane of neuroblastoma cells grown in suspension culture were about 7.3 +/- 0.8 microA/microF and from 37 +/- 5.2 microA/microF to 54.7 +/- 3.6 microA/microF of differentiated cells at different terms of cultivation. Densities of calcium currents in the membrane of cells grown in suspension were about 1.4 +/- 0.2 microA/microF, while in differentiated cells they ranged from 1.1 +/- 0.2 to 2.8 +/- 0.4 microA/microF at different terms of cultivation. Induction or reduction of differentiation by varying pH of the culture medium produced reciprocal changes in densities of sodium and calcium channels.

UR - http://www.scopus.com/inward/record.url?scp=0022566747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022566747&partnerID=8YFLogxK

M3 - Article

C2 - 2423892

AN - SCOPUS:0022566747

VL - 18

SP - 207

EP - 214

JO - Neirofiziologiya

JF - Neirofiziologiya

SN - 0028-2561

IS - 2

ER -