SOD2-mediated effects induced by WR1065 and low-dose ionizing radiation on micronucleus formation in RKO human colon carcinoma cells

Jeffrey S. Murley, Yasushi Kataoka, Richard C. Miller, Jian-Jian Li, Gayle Woloschak, David J. Grdina

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

RKO36 cells exposed to either WR1065 or 10 cGy X rays show elevated SOD2 gene expression and SOD2 enzymatic activity. Cells challenged at this time with 2 Gy exhibit enhanced radiation resistance. This phenomenon has been identified as a delayed radioprotective effect or an adaptive response when induced by thiols or low-dose radiation, respectively. In this study we investigated the relative effectiveness of both WR1065 and low-dose radiation in reducing the incidence of radiation-induced micronucleus formation in binucleated RKO36 human colon carcinoma cells. The role of SOD2 in this process was assessed by measuring changes in enzymatic activity as a function of the inducing agent used, the level of protection afforded, and the inhibitory effects of short interfering RNA (SOD2 siRNA). Both WR1065 and 10 cGy X rays effectively induced a greater than threefold elevation in SOD2 activity 24 h after exposure. Cells irradiated at this time with 2 Gy exhibited a significant resistance to micronucleus formation (P < 0.05; Student's two-tailed t test). This protective effect was significantly inhibited in cells transfected with SOD2 siRNA. SOD2 played an important role in the adaptive/delayed radioprotective response by inhibiting the initiation of a superoxide anion-induced ROS cascade leading to enhanced mitochondrial and nuclear damages.

Original languageEnglish (US)
Pages (from-to)57-65
Number of pages9
JournalRadiation Research
Volume175
Issue number1
DOIs
StatePublished - Jan 2011

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Ionizing Radiation
ionizing radiation
Colon
cancer
Carcinoma
dosage
Radiation
Small Interfering RNA
radiation
cells
inorganic peroxides
gene expression
radiation tolerance
thiols
students
X-Rays
cascades
x rays
incidence
anions

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Biophysics
  • Radiation

Cite this

SOD2-mediated effects induced by WR1065 and low-dose ionizing radiation on micronucleus formation in RKO human colon carcinoma cells. / Murley, Jeffrey S.; Kataoka, Yasushi; Miller, Richard C.; Li, Jian-Jian; Woloschak, Gayle; Grdina, David J.

In: Radiation Research, Vol. 175, No. 1, 01.2011, p. 57-65.

Research output: Contribution to journalArticle

Murley, Jeffrey S. ; Kataoka, Yasushi ; Miller, Richard C. ; Li, Jian-Jian ; Woloschak, Gayle ; Grdina, David J. / SOD2-mediated effects induced by WR1065 and low-dose ionizing radiation on micronucleus formation in RKO human colon carcinoma cells. In: Radiation Research. 2011 ; Vol. 175, No. 1. pp. 57-65.
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