SNP-based codominant markers for a recessive gene conferring resistance to corky root rot (Rhizomonas suberifaciens) in lettuce (Lactuca sativa)

Santiago Moreno-Vázquez, Oswaldo E. Ochoa, Nanne Faber, Shiaoman Chao, Jeanne M E Jacobs, Brigitte Maisonneuve, Richard V. Kesseli, Richard W Michelmore

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The analysis of F2 progeny and derived F3 families of Lactuca sativa segregating for resistance to corky root rot caused by Rhizomonas suberifaciens permitted the identification of restriction fragment length polymorphism (RFLP) and single nucleotide polymorphism (SNP) markers linked to the recessive resistance gene cor. PCR-based markers were identified by bulked segregant analysis (BSA). Allele-specific primers were generally designed with the 3′ terminal base coinciding with an SNP, matching one of the alleles and mismatching the other, and with an additional subterminal 3′ base mismatching both alleles. Codominant, robust, and inexpensive molecular markers were obtained that used standardized PCR conditions. Some of the markers could be analyzed in multiple Lactuca mapping populations that did not segregate for disease resistance allowing the cor locus to be located on several maps. The consistent low density of markers around cor in these maps suggests that cor may be in an area with an elevated rate of recombination. Evaluation of these markers in a large sample of cultivars and landraces identified pairs of flanking polymorphic markers that can be used for marker-assisted selection of corky root resistance.

Original languageEnglish (US)
Pages (from-to)1059-1069
Number of pages11
JournalGenome
Volume46
Issue number6
DOIs
StatePublished - Dec 2003

Fingerprint

Sphingomonas suberifaciens
Recessive Genes
Lettuce
recessive genes
Lactuca sativa
root rot
lettuce
single nucleotide polymorphism
Single Nucleotide Polymorphism
Alleles
alleles
Lactuca
Polymerase Chain Reaction
Disease Resistance
marker-assisted selection
Restriction Fragment Length Polymorphisms
landraces
Genetic Recombination
disease resistance
restriction fragment length polymorphism

Keywords

  • Genetic map
  • Marker-assisted selection (MAS)
  • Resistance gene
  • Sequence characterized amplified region (SCAR)
  • Single nucleotide polymorphism (SNP)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biotechnology
  • Genetics
  • Genetics(clinical)

Cite this

Moreno-Vázquez, S., Ochoa, O. E., Faber, N., Chao, S., Jacobs, J. M. E., Maisonneuve, B., ... Michelmore, R. W. (2003). SNP-based codominant markers for a recessive gene conferring resistance to corky root rot (Rhizomonas suberifaciens) in lettuce (Lactuca sativa). Genome, 46(6), 1059-1069. https://doi.org/10.1139/g03-073

SNP-based codominant markers for a recessive gene conferring resistance to corky root rot (Rhizomonas suberifaciens) in lettuce (Lactuca sativa). / Moreno-Vázquez, Santiago; Ochoa, Oswaldo E.; Faber, Nanne; Chao, Shiaoman; Jacobs, Jeanne M E; Maisonneuve, Brigitte; Kesseli, Richard V.; Michelmore, Richard W.

In: Genome, Vol. 46, No. 6, 12.2003, p. 1059-1069.

Research output: Contribution to journalArticle

Moreno-Vázquez, S, Ochoa, OE, Faber, N, Chao, S, Jacobs, JME, Maisonneuve, B, Kesseli, RV & Michelmore, RW 2003, 'SNP-based codominant markers for a recessive gene conferring resistance to corky root rot (Rhizomonas suberifaciens) in lettuce (Lactuca sativa)', Genome, vol. 46, no. 6, pp. 1059-1069. https://doi.org/10.1139/g03-073
Moreno-Vázquez, Santiago ; Ochoa, Oswaldo E. ; Faber, Nanne ; Chao, Shiaoman ; Jacobs, Jeanne M E ; Maisonneuve, Brigitte ; Kesseli, Richard V. ; Michelmore, Richard W. / SNP-based codominant markers for a recessive gene conferring resistance to corky root rot (Rhizomonas suberifaciens) in lettuce (Lactuca sativa). In: Genome. 2003 ; Vol. 46, No. 6. pp. 1059-1069.
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