Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways

Masayo Morishita, Rima Mendonsa, Jennifer Wright, JoAnne Engebrecht

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Vesicular traffic is essential for sporulation in Saccharomyces cerevisiae. The Golgi-associated retrograde protein (GARP) tethering complex is required for retrograde traffic from both the early and late endosomes to the Golgi. Analyses of GARP complex mutants in sporulation reveal defects in meiotic progression and spore formation. In contrast, inactivation of the retromer complex, which mediates vesicle budding and cargo selection from the late endosome, or Snx4p, which is involved in retrieval of proteins from the early endosome, has little effect on sporulation. A retromer GARP double mutant is defective in the formation of the prospore membrane (PSM) that surrounds the haploid nuclei. In the retromer GARP double mutant, PSM precursor vesicles carrying the cargo, Dtr1p, are transported to the spindle pole body (SPB), where PSM formation is initiated. However, the v-SNARE Snc1p is not transported to the SPB in the double mutant, suggesting that the defect in PSM formation is because of the failure to retrieve Snc1p, and perhaps other proteins, from the endosomal pathway. Taken together, these results indicate that retrograde trafficking from the endosome is essential for sporulation by retrieving molecules important for PSM and spore wall formation.

Original languageEnglish (US)
Pages (from-to)1231-1245
Number of pages15
JournalTraffic
Volume8
Issue number9
DOIs
StatePublished - Sep 2007

Fingerprint

SNARE Proteins
Yeast
Endosomes
Yeasts
Membranes
Mutant Proteins
Spindle Pole Bodies
Proteins
Spores
Poles
Haploidy
Defects
Saccharomyces cerevisiae
Molecules

Keywords

  • Endosome
  • GARP
  • Prospore membrane
  • Retromer
  • Snc1p
  • Sporulation
  • Yeast

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Structural Biology
  • Molecular Biology
  • Genetics

Cite this

Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways. / Morishita, Masayo; Mendonsa, Rima; Wright, Jennifer; Engebrecht, JoAnne.

In: Traffic, Vol. 8, No. 9, 09.2007, p. 1231-1245.

Research output: Contribution to journalArticle

Morishita, Masayo ; Mendonsa, Rima ; Wright, Jennifer ; Engebrecht, JoAnne. / Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways. In: Traffic. 2007 ; Vol. 8, No. 9. pp. 1231-1245.
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