Abstract
Vesicular traffic is essential for sporulation in Saccharomyces cerevisiae. The Golgi-associated retrograde protein (GARP) tethering complex is required for retrograde traffic from both the early and late endosomes to the Golgi. Analyses of GARP complex mutants in sporulation reveal defects in meiotic progression and spore formation. In contrast, inactivation of the retromer complex, which mediates vesicle budding and cargo selection from the late endosome, or Snx4p, which is involved in retrieval of proteins from the early endosome, has little effect on sporulation. A retromer GARP double mutant is defective in the formation of the prospore membrane (PSM) that surrounds the haploid nuclei. In the retromer GARP double mutant, PSM precursor vesicles carrying the cargo, Dtr1p, are transported to the spindle pole body (SPB), where PSM formation is initiated. However, the v-SNARE Snc1p is not transported to the SPB in the double mutant, suggesting that the defect in PSM formation is because of the failure to retrieve Snc1p, and perhaps other proteins, from the endosomal pathway. Taken together, these results indicate that retrograde trafficking from the endosome is essential for sporulation by retrieving molecules important for PSM and spore wall formation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1231-1245 |
| Number of pages | 15 |
| Journal | Traffic |
| Volume | 8 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2007 |
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Keywords
- Endosome
- GARP
- Prospore membrane
- Retromer
- Snc1p
- Sporulation
- Yeast
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Structural Biology
- Molecular Biology
- Genetics
Cite this
Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways. / Morishita, Masayo; Mendonsa, Rima; Wright, Jennifer; Engebrecht, JoAnne.
In: Traffic, Vol. 8, No. 9, 09.2007, p. 1231-1245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Snc1p v-SNARE transport to the prospore membrane during yeast sporulation is dependent on endosomal retrieval pathways
AU - Morishita, Masayo
AU - Mendonsa, Rima
AU - Wright, Jennifer
AU - Engebrecht, JoAnne
PY - 2007/9
Y1 - 2007/9
N2 - Vesicular traffic is essential for sporulation in Saccharomyces cerevisiae. The Golgi-associated retrograde protein (GARP) tethering complex is required for retrograde traffic from both the early and late endosomes to the Golgi. Analyses of GARP complex mutants in sporulation reveal defects in meiotic progression and spore formation. In contrast, inactivation of the retromer complex, which mediates vesicle budding and cargo selection from the late endosome, or Snx4p, which is involved in retrieval of proteins from the early endosome, has little effect on sporulation. A retromer GARP double mutant is defective in the formation of the prospore membrane (PSM) that surrounds the haploid nuclei. In the retromer GARP double mutant, PSM precursor vesicles carrying the cargo, Dtr1p, are transported to the spindle pole body (SPB), where PSM formation is initiated. However, the v-SNARE Snc1p is not transported to the SPB in the double mutant, suggesting that the defect in PSM formation is because of the failure to retrieve Snc1p, and perhaps other proteins, from the endosomal pathway. Taken together, these results indicate that retrograde trafficking from the endosome is essential for sporulation by retrieving molecules important for PSM and spore wall formation.
AB - Vesicular traffic is essential for sporulation in Saccharomyces cerevisiae. The Golgi-associated retrograde protein (GARP) tethering complex is required for retrograde traffic from both the early and late endosomes to the Golgi. Analyses of GARP complex mutants in sporulation reveal defects in meiotic progression and spore formation. In contrast, inactivation of the retromer complex, which mediates vesicle budding and cargo selection from the late endosome, or Snx4p, which is involved in retrieval of proteins from the early endosome, has little effect on sporulation. A retromer GARP double mutant is defective in the formation of the prospore membrane (PSM) that surrounds the haploid nuclei. In the retromer GARP double mutant, PSM precursor vesicles carrying the cargo, Dtr1p, are transported to the spindle pole body (SPB), where PSM formation is initiated. However, the v-SNARE Snc1p is not transported to the SPB in the double mutant, suggesting that the defect in PSM formation is because of the failure to retrieve Snc1p, and perhaps other proteins, from the endosomal pathway. Taken together, these results indicate that retrograde trafficking from the endosome is essential for sporulation by retrieving molecules important for PSM and spore wall formation.
KW - Endosome
KW - GARP
KW - Prospore membrane
KW - Retromer
KW - Snc1p
KW - Sporulation
KW - Yeast
UR - http://www.scopus.com/inward/record.url?scp=34547839587&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34547839587&partnerID=8YFLogxK
U2 - 10.1111/j.1600-0854.2007.00606.x
DO - 10.1111/j.1600-0854.2007.00606.x
M3 - Article
C2 - 17645731
AN - SCOPUS:34547839587
VL - 8
SP - 1231
EP - 1245
JO - Traffic
JF - Traffic
SN - 1398-9219
IS - 9
ER -