Abstract
The SMRT and NCoR corepressors bind to, and mediate transcriptional repression by, many nuclear receptors. Both SMRT and NCoR are expressed by alternative mRNA splicing, generating a series of structurally and functionally distinct corepressor " variants" We report that a splice variant of SMRT, SMRTε, recognizes a restricted subset of nuclear receptors. Unlike the other corepressor variants characterized, SMRTε possesses only a single receptor interaction domain (RID) and exhibits an unusual specificity for a subset of nuclear receptors that includes the retinoic acid receptors (RARs). The ability of the single RID in SMRTε to efficiently interact with RARs appears to be enhanced by a recently recognized β-strand/β-strand interaction between corepressor and receptor. We suggest that alternative mRNA splicing of corepressors can restrict their function to specific nuclear receptor partnerships, and we propose that this may serve to customize the transcriptional repression properties of different cell types for different biological purposes.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 306-316 |
| Number of pages | 11 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 351 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 4 2012 |
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Keywords
- β-Strand
- Alternative-mRNA splicing
- Corepressors
- Nuclear receptors
- Retinoic acid receptors
- SMRT
ASJC Scopus subject areas
- Endocrinology
- Molecular Biology
- Biochemistry
Cite this
SMRTε, a corepressor variant, interacts with a restricted subset of nuclear receptors, including the retinoic acid receptors α and β. / Mengeling, Brenda J.; Goodson, Michael L.; Bourguet, William; Privalsky, Martin L.
In: Molecular and Cellular Endocrinology, Vol. 351, No. 2, 04.04.2012, p. 306-316.Research output: Contribution to journal › Article
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TY - JOUR
T1 - SMRTε, a corepressor variant, interacts with a restricted subset of nuclear receptors, including the retinoic acid receptors α and β
AU - Mengeling, Brenda J.
AU - Goodson, Michael L.
AU - Bourguet, William
AU - Privalsky, Martin L.
PY - 2012/4/4
Y1 - 2012/4/4
N2 - The SMRT and NCoR corepressors bind to, and mediate transcriptional repression by, many nuclear receptors. Both SMRT and NCoR are expressed by alternative mRNA splicing, generating a series of structurally and functionally distinct corepressor " variants" We report that a splice variant of SMRT, SMRTε, recognizes a restricted subset of nuclear receptors. Unlike the other corepressor variants characterized, SMRTε possesses only a single receptor interaction domain (RID) and exhibits an unusual specificity for a subset of nuclear receptors that includes the retinoic acid receptors (RARs). The ability of the single RID in SMRTε to efficiently interact with RARs appears to be enhanced by a recently recognized β-strand/β-strand interaction between corepressor and receptor. We suggest that alternative mRNA splicing of corepressors can restrict their function to specific nuclear receptor partnerships, and we propose that this may serve to customize the transcriptional repression properties of different cell types for different biological purposes.
AB - The SMRT and NCoR corepressors bind to, and mediate transcriptional repression by, many nuclear receptors. Both SMRT and NCoR are expressed by alternative mRNA splicing, generating a series of structurally and functionally distinct corepressor " variants" We report that a splice variant of SMRT, SMRTε, recognizes a restricted subset of nuclear receptors. Unlike the other corepressor variants characterized, SMRTε possesses only a single receptor interaction domain (RID) and exhibits an unusual specificity for a subset of nuclear receptors that includes the retinoic acid receptors (RARs). The ability of the single RID in SMRTε to efficiently interact with RARs appears to be enhanced by a recently recognized β-strand/β-strand interaction between corepressor and receptor. We suggest that alternative mRNA splicing of corepressors can restrict their function to specific nuclear receptor partnerships, and we propose that this may serve to customize the transcriptional repression properties of different cell types for different biological purposes.
KW - β-Strand
KW - Alternative-mRNA splicing
KW - Corepressors
KW - Nuclear receptors
KW - Retinoic acid receptors
KW - SMRT
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UR - http://www.scopus.com/inward/citedby.url?scp=84857140413&partnerID=8YFLogxK
U2 - 10.1016/j.mce.2012.01.006
DO - 10.1016/j.mce.2012.01.006
M3 - Article
C2 - 22266197
AN - SCOPUS:84857140413
VL - 351
SP - 306
EP - 316
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
SN - 0303-7207
IS - 2
ER -