Smoking increases proteolytic activity in the human abdominal aorta

Jose R. Parra, Robert A. Cambria, Julie A. Freischlag, Gary R. Seabrook, Jonathan B. Towne

Research output: Contribution to journalArticle

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Abstract

To determine the mechanism of smoking on the development of abdominal aortic aneurysms, the authors evaluated the proteolytic activity and quantity of matrix metalloproteinase enzymes in human aortic tissue. Abdominal aortic specimens of normal caliber were harvested from 13 organ donors, eight of whom had a history of smoking. Tissue samples were snap frozen in liquid nitrogen, pulverized, resuspended in a protein isolation buffer, and centrifuged to obtain the protein fraction. Gelatin substrate zymography was performed to determine proteolytic activity. Western immunoblotting using monoclonal antibodies directed against matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) was also performed. A significant increase in proteolytic activity was noted on zymography at 68 kilodaltons (kDa) in samples from smoking patients. Proteolytic activity at 96 kDa was not different between smokers and nonsmokers. No differences in the quantity of MMP-2 or MMP-9 were noted on immunoblots between the smoking and nonsmoking groups. These data establish a link between smoking and increased proteolytic activity in the human aortic wall and implicate smoking in the etiology of aortic aneurysms.

Original languageEnglish (US)
Pages (from-to)617-622
Number of pages6
JournalVascular Surgery
Volume32
Issue number6
StatePublished - 1998
Externally publishedYes

Fingerprint

Abdominal Aorta
Human Activities
Smoking
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Aortic Aneurysm
Abdominal Aortic Aneurysm
Gelatin
Matrix Metalloproteinases
Buffers
Proteins
Nitrogen
Western Blotting
Monoclonal Antibodies
Tissue Donors
Enzymes

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Parra, J. R., Cambria, R. A., Freischlag, J. A., Seabrook, G. R., & Towne, J. B. (1998). Smoking increases proteolytic activity in the human abdominal aorta. Vascular Surgery, 32(6), 617-622.

Smoking increases proteolytic activity in the human abdominal aorta. / Parra, Jose R.; Cambria, Robert A.; Freischlag, Julie A.; Seabrook, Gary R.; Towne, Jonathan B.

In: Vascular Surgery, Vol. 32, No. 6, 1998, p. 617-622.

Research output: Contribution to journalArticle

Parra, JR, Cambria, RA, Freischlag, JA, Seabrook, GR & Towne, JB 1998, 'Smoking increases proteolytic activity in the human abdominal aorta', Vascular Surgery, vol. 32, no. 6, pp. 617-622.
Parra JR, Cambria RA, Freischlag JA, Seabrook GR, Towne JB. Smoking increases proteolytic activity in the human abdominal aorta. Vascular Surgery. 1998;32(6):617-622.
Parra, Jose R. ; Cambria, Robert A. ; Freischlag, Julie A. ; Seabrook, Gary R. ; Towne, Jonathan B. / Smoking increases proteolytic activity in the human abdominal aorta. In: Vascular Surgery. 1998 ; Vol. 32, No. 6. pp. 617-622.
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