Smoking affects gene expression in blood of patients with ischemic stroke

Xiyuan Cheng, Eva Ferino, Heather Hull, Glen C. Jickling, Bradley Ander, Boryana Stamova, Frank R Sharp

Research output: Contribution to journalArticle

Abstract

Objective: Though cigarette smoking (CS) is a well-known risk factor for ischemic stroke (IS), there is no data on how CS affects the blood transcriptome in IS patients. Methods: We recruited IS-current smokers (IS-SM), IS-never smokers (IS-NSM), control-smokers (C-SM), and control-never smokers (C-NSM). mRNA expression was assessed on HTA-2.0 microarrays and unique as well as commonly expressed genes identified for IS-SM versus IS-NSM and C-SM versus C-NSM. Results: One hundred and fifty-eight genes were differentially expressed in IS-SM versus IS-NSM; 100 genes were differentially expressed in C-SM versus C-NSM; and 10 genes were common to both IS-SM and C-SM (P < 0.01; |fold change| ≥ 1.2). Functional pathway analysis showed the 158 IS-SM-regulated genes were associated with T-cell receptor, cytokine–cytokine receptor, chemokine, adipocytokine, tight junction, Jak-STAT, ubiquitin-mediated proteolysis, and adherens junction signaling. IS-SM showed more altered genes and functional networks than C-SM. Interpretation: We propose some of the 10 genes that are elevated in both IS-SM and C-SM (GRP15, LRRN3, CLDND1, ICOS, GCNT4, VPS13A, DAP3, SNORA54, HIST1H1D, and SCARNA6) might contribute to increased risk of stroke in current smokers, and some genes expressed by blood leukocytes and platelets after stroke in smokers might contribute to worse stroke outcomes that occur in smokers.

Original languageEnglish (US)
JournalAnnals of Clinical and Translational Neurology
DOIs
StateAccepted/In press - Jan 1 2019

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Smoking
Stroke
Gene Expression
Genes
Adherens Junctions
Adipokines
Tight Junctions
Gene Regulatory Networks
Chemokine Receptors
Ubiquitin
T-Cell Antigen Receptor
Transcriptome
Proteolysis
Leukocytes
Blood Platelets
Messenger RNA

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Neurology

Cite this

Smoking affects gene expression in blood of patients with ischemic stroke. / Cheng, Xiyuan; Ferino, Eva; Hull, Heather; Jickling, Glen C.; Ander, Bradley; Stamova, Boryana; Sharp, Frank R.

In: Annals of Clinical and Translational Neurology, 01.01.2019.

Research output: Contribution to journalArticle

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AU - Ferino, Eva

AU - Hull, Heather

AU - Jickling, Glen C.

AU - Ander, Bradley

AU - Stamova, Boryana

AU - Sharp, Frank R

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