Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay

Arnold Chen, Royal Wang, Candace R S Bever, Siyuan Xing, Bruce D. Hammock, Tingrui Pan

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The emerging technologies on mobile-based diagnosis and bioanalytical detection have enabled powerful laboratory assays such as enzyme-linked immunosorbent assay (ELISA) to be conducted in field-use lab-on-a-chip devices. In this paper, we present a low-cost universal serial bus (USB)-interfaced mobile platform to perform microfluidic ELISA operations in detecting the presence and concentrations of BDE-47 (2,2,4,4-tetrabromodiphenyl ether), an environmental contaminant found in our food supply with adverse health impact. Our point-of-care diagnostic device utilizes flexible interdigitated carbon black electrodes to convert electric current into a microfluidic pump via gas bubble expansion during electrolytic reaction. The micropump receives power from a mobile phone and transports BDE-47 analytes through the microfluidic device conducting competitive ELISA. Using variable domain of heavy chain antibodies (commonly referred to as single domain antibodies or Nanobodies), the proposed device is sensitive for a BDE-47 concentration range of 10-3-104μg/l, with a comparable performance to that uses a standard competitive ELISA protocol. It is anticipated that the potential impact in mobile detection of health and environmental contaminants will prove beneficial to our community and low-resource environments.

Original languageEnglish (US)
Article number064101
JournalBiomicrofluidics
Volume8
Issue number6
DOIs
StatePublished - Nov 5 2014

Fingerprint

lab-on-a-chip devices
Lab-on-a-chip
Immunosorbents
Smartphones
enzymes
Assays
Enzymes
Single-Domain Antibodies
Enzyme-Linked Immunosorbent Assay
Equipment and Supplies
Microfluidics
Lab-On-A-Chip Devices
antibodies
health
contaminants
Antibodies
Point-of-Care Systems
Soot
Cell Phones
Food Supply

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Condensed Matter Physics
  • Materials Science(all)
  • Physical and Theoretical Chemistry

Cite this

Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay. / Chen, Arnold; Wang, Royal; Bever, Candace R S; Xing, Siyuan; Hammock, Bruce D.; Pan, Tingrui.

In: Biomicrofluidics, Vol. 8, No. 6, 064101, 05.11.2014.

Research output: Contribution to journalArticle

Chen, Arnold ; Wang, Royal ; Bever, Candace R S ; Xing, Siyuan ; Hammock, Bruce D. ; Pan, Tingrui. / Smartphone-interfaced lab-on-a-chip devices for field-deployable enzyme-linked immunosorbent assay. In: Biomicrofluidics. 2014 ; Vol. 8, No. 6.
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