Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201

Effect on Brain Tissue Oxygenation

Geoffrey T. Manley, J. Claude Hemphill, Diane Morabito, Vanessa Erickson, John Holcroft, Nakita Derugin, M. Margaret Knudson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

OBJECTIVES: To investigate the effects of small-volume resuscitation with a hemoglobin based oxygen carrier on brain tissue oxygen tension (PbrO 2) in hemorrhaged swine. METHODS: Clark-type electrodes were inserted into the brain tissue of 6 swine to measure PbrO2 directly. Swine were hemorrhaged to a MAP of 40 mm Hg for 20 minutes. Resuscitation was performed with a bolus infusion of HBOC-201 (6 cc/kg; Biopure Corp.) and high-flow oxygen (100%). Swine were observed for an additional 2 hours. RESULTS: PbrO2 prior to hemorrhage was 48.7 ± 4.7 mm Hg with 100% inspired oxygen. PbrO2 rapidly declined to 7.6 ± 5.3 mm Hg in response to hemorrhage. Small-volume resuscitation with HBOC-201 and high-flow oxygen resulted in a significant increase (p<0.001) in PbrO2 to 44.6 ± 8.1 mm Hg. MAP was also significantly increased to 84% of baseline. These elevations were sustained during the observation period. CONCLUSIONS: Resuscitation with HBOC-201 can restore and sustain cerebral oxygenation and MAP. These results suggest that a small-volume bolus of HBOC-201 may provide adequate oxygen and pressure support during the initial management of hemorrhage.

Original languageEnglish (US)
Pages (from-to)311-317
Number of pages7
JournalAdvances in Experimental Medicine and Biology
Volume530
StatePublished - 2003

Fingerprint

Resuscitation
Blood Substitutes
Oxygenation
Brain
Tissue
Oxygen
Swine
Hemorrhage
HBOC 201
Electrodes
Hemoglobins
Observation
Pressure

Keywords

  • Blood substitutes
  • Cerebral oxygenation
  • Cerebral resuscitation
  • Hemorrhage

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Manley, G. T., Hemphill, J. C., Morabito, D., Erickson, V., Holcroft, J., Derugin, N., & Knudson, M. M. (2003). Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201: Effect on Brain Tissue Oxygenation. Advances in Experimental Medicine and Biology, 530, 311-317.

Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201 : Effect on Brain Tissue Oxygenation. / Manley, Geoffrey T.; Hemphill, J. Claude; Morabito, Diane; Erickson, Vanessa; Holcroft, John; Derugin, Nakita; Knudson, M. Margaret.

In: Advances in Experimental Medicine and Biology, Vol. 530, 2003, p. 311-317.

Research output: Contribution to journalArticle

Manley, GT, Hemphill, JC, Morabito, D, Erickson, V, Holcroft, J, Derugin, N & Knudson, MM 2003, 'Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201: Effect on Brain Tissue Oxygenation', Advances in Experimental Medicine and Biology, vol. 530, pp. 311-317.
Manley, Geoffrey T. ; Hemphill, J. Claude ; Morabito, Diane ; Erickson, Vanessa ; Holcroft, John ; Derugin, Nakita ; Knudson, M. Margaret. / Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201 : Effect on Brain Tissue Oxygenation. In: Advances in Experimental Medicine and Biology. 2003 ; Vol. 530. pp. 311-317.
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