Abstract
Aim: To investigate the effect of synthetic small interfering RNA (siRNA) targeting β-arrestin2 on the apoptosis of hepatic stellate cells (HSC) in vitro. Methods Synthetic siRNA targeting β-arrestin2 was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control, respectively. After incubation with siRNA, total RNA and protein of HSC-T6 cells were extracted. The expression of β-arrestin2 gene and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HSC-T6 apoptosis was measured by flow cytometry. The expression of Bcl-2 and Bax were determined by Western blot. Results: After HSC-T6 cells were transfected with β-arrestin2 siRNA, the level of β-arrestin2 mRNA and protein expression was decresed by 70% ±1.76% (P < 0.01) and 68.43% ± 2.88% (P < 0.01) as compared with the control group. Bcl-2 expression was also inhibited by 32.58% ±3.46% (P < 0.01) , while Bax expression was increased by 38.00% ±3.72% (P < 0.01). The apoptosis rate of HSC-T6 transfected with β-arrestin2 siRNA was 37.5% , which was significantly higher than control. Conclusion Inhibition of β-arrestin2 by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by promoting apoptosis of HSC.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 612-616 |
| Number of pages | 5 |
| Journal | Chinese Pharmacological Bulletin |
| Volume | 28 |
| Issue number | 5 |
| DOIs | |
| State | Published - Jan 1 2012 |
| Externally published | Yes |
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Keywords
- Apoptosis
- Bax
- Bcl-2
- Hepatic fibrosis
- Hepatic stellate cells
- Small interfering RNA
- β-arrestin2
ASJC Scopus subject areas
- Pharmacology
Cite this
Small interfering RNA targeting β-arrestin2 promoted apoptosis of hepatic stellate cells. / Song, Yang; Sun, Wu Yi; Hu, Shan Shan; Wang, Qingtong; Wei, Wei.
In: Chinese Pharmacological Bulletin, Vol. 28, No. 5, 01.01.2012, p. 612-616.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Small interfering RNA targeting β-arrestin2 promoted apoptosis of hepatic stellate cells
AU - Song, Yang
AU - Sun, Wu Yi
AU - Hu, Shan Shan
AU - Wang, Qingtong
AU - Wei, Wei
PY - 2012/1/1
Y1 - 2012/1/1
N2 - Aim: To investigate the effect of synthetic small interfering RNA (siRNA) targeting β-arrestin2 on the apoptosis of hepatic stellate cells (HSC) in vitro. Methods Synthetic siRNA targeting β-arrestin2 was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control, respectively. After incubation with siRNA, total RNA and protein of HSC-T6 cells were extracted. The expression of β-arrestin2 gene and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HSC-T6 apoptosis was measured by flow cytometry. The expression of Bcl-2 and Bax were determined by Western blot. Results: After HSC-T6 cells were transfected with β-arrestin2 siRNA, the level of β-arrestin2 mRNA and protein expression was decresed by 70% ±1.76% (P < 0.01) and 68.43% ± 2.88% (P < 0.01) as compared with the control group. Bcl-2 expression was also inhibited by 32.58% ±3.46% (P < 0.01) , while Bax expression was increased by 38.00% ±3.72% (P < 0.01). The apoptosis rate of HSC-T6 transfected with β-arrestin2 siRNA was 37.5% , which was significantly higher than control. Conclusion Inhibition of β-arrestin2 by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by promoting apoptosis of HSC.
AB - Aim: To investigate the effect of synthetic small interfering RNA (siRNA) targeting β-arrestin2 on the apoptosis of hepatic stellate cells (HSC) in vitro. Methods Synthetic siRNA targeting β-arrestin2 was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control, respectively. After incubation with siRNA, total RNA and protein of HSC-T6 cells were extracted. The expression of β-arrestin2 gene and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HSC-T6 apoptosis was measured by flow cytometry. The expression of Bcl-2 and Bax were determined by Western blot. Results: After HSC-T6 cells were transfected with β-arrestin2 siRNA, the level of β-arrestin2 mRNA and protein expression was decresed by 70% ±1.76% (P < 0.01) and 68.43% ± 2.88% (P < 0.01) as compared with the control group. Bcl-2 expression was also inhibited by 32.58% ±3.46% (P < 0.01) , while Bax expression was increased by 38.00% ±3.72% (P < 0.01). The apoptosis rate of HSC-T6 transfected with β-arrestin2 siRNA was 37.5% , which was significantly higher than control. Conclusion Inhibition of β-arrestin2 by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by promoting apoptosis of HSC.
KW - Apoptosis
KW - Bax
KW - Bcl-2
KW - Hepatic fibrosis
KW - Hepatic stellate cells
KW - Small interfering RNA
KW - β-arrestin2
UR - http://www.scopus.com/inward/record.url?scp=84864115553&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84864115553&partnerID=8YFLogxK
U2 - 10.3969/j.issn.1001-1978.2012.05.006
DO - 10.3969/j.issn.1001-1978.2012.05.006
M3 - Article
AN - SCOPUS:84864115553
VL - 28
SP - 612
EP - 616
JO - Chinese Pharmacological Bulletin
JF - Chinese Pharmacological Bulletin
SN - 1001-1978
IS - 5
ER -