Small interfering RNA targeting β-arrestin2 promoted apoptosis of hepatic stellate cells

Yang Song, Wu Yi Sun, Shan Shan Hu, Qingtong Wang, Wei Wei

Research output: Contribution to journalArticle

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Abstract

Aim: To investigate the effect of synthetic small interfering RNA (siRNA) targeting β-arrestin2 on the apoptosis of hepatic stellate cells (HSC) in vitro. Methods Synthetic siRNA targeting β-arrestin2 was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control, respectively. After incubation with siRNA, total RNA and protein of HSC-T6 cells were extracted. The expression of β-arrestin2 gene and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HSC-T6 apoptosis was measured by flow cytometry. The expression of Bcl-2 and Bax were determined by Western blot. Results: After HSC-T6 cells were transfected with β-arrestin2 siRNA, the level of β-arrestin2 mRNA and protein expression was decresed by 70% ±1.76% (P < 0.01) and 68.43% ± 2.88% (P < 0.01) as compared with the control group. Bcl-2 expression was also inhibited by 32.58% ±3.46% (P < 0.01) , while Bax expression was increased by 38.00% ±3.72% (P < 0.01). The apoptosis rate of HSC-T6 transfected with β-arrestin2 siRNA was 37.5% , which was significantly higher than control. Conclusion Inhibition of β-arrestin2 by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by promoting apoptosis of HSC.

Original languageEnglish (US)
Pages (from-to)612-616
Number of pages5
JournalChinese Pharmacological Bulletin
Volume28
Issue number5
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

Fingerprint

Hepatic Stellate Cells
Small Interfering RNA
Apoptosis
Transfection
Western Blotting
Proteins
Reverse Transcription
Flow Cytometry
Fibrosis
RNA
Polymerase Chain Reaction
Control Groups
Messenger RNA
Liver

Keywords

  • Apoptosis
  • Bax
  • Bcl-2
  • Hepatic fibrosis
  • Hepatic stellate cells
  • Small interfering RNA
  • β-arrestin2

ASJC Scopus subject areas

  • Pharmacology

Cite this

Small interfering RNA targeting β-arrestin2 promoted apoptosis of hepatic stellate cells. / Song, Yang; Sun, Wu Yi; Hu, Shan Shan; Wang, Qingtong; Wei, Wei.

In: Chinese Pharmacological Bulletin, Vol. 28, No. 5, 01.01.2012, p. 612-616.

Research output: Contribution to journalArticle

Song, Yang ; Sun, Wu Yi ; Hu, Shan Shan ; Wang, Qingtong ; Wei, Wei. / Small interfering RNA targeting β-arrestin2 promoted apoptosis of hepatic stellate cells. In: Chinese Pharmacological Bulletin. 2012 ; Vol. 28, No. 5. pp. 612-616.
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abstract = "Aim: To investigate the effect of synthetic small interfering RNA (siRNA) targeting β-arrestin2 on the apoptosis of hepatic stellate cells (HSC) in vitro. Methods Synthetic siRNA targeting β-arrestin2 was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control, respectively. After incubation with siRNA, total RNA and protein of HSC-T6 cells were extracted. The expression of β-arrestin2 gene and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HSC-T6 apoptosis was measured by flow cytometry. The expression of Bcl-2 and Bax were determined by Western blot. Results: After HSC-T6 cells were transfected with β-arrestin2 siRNA, the level of β-arrestin2 mRNA and protein expression was decresed by 70{\%} ±1.76{\%} (P < 0.01) and 68.43{\%} ± 2.88{\%} (P < 0.01) as compared with the control group. Bcl-2 expression was also inhibited by 32.58{\%} ±3.46{\%} (P < 0.01) , while Bax expression was increased by 38.00{\%} ±3.72{\%} (P < 0.01). The apoptosis rate of HSC-T6 transfected with β-arrestin2 siRNA was 37.5{\%} , which was significantly higher than control. Conclusion Inhibition of β-arrestin2 by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by promoting apoptosis of HSC.",
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T1 - Small interfering RNA targeting β-arrestin2 promoted apoptosis of hepatic stellate cells

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AU - Hu, Shan Shan

AU - Wang, Qingtong

AU - Wei, Wei

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N2 - Aim: To investigate the effect of synthetic small interfering RNA (siRNA) targeting β-arrestin2 on the apoptosis of hepatic stellate cells (HSC) in vitro. Methods Synthetic siRNA targeting β-arrestin2 was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control, respectively. After incubation with siRNA, total RNA and protein of HSC-T6 cells were extracted. The expression of β-arrestin2 gene and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HSC-T6 apoptosis was measured by flow cytometry. The expression of Bcl-2 and Bax were determined by Western blot. Results: After HSC-T6 cells were transfected with β-arrestin2 siRNA, the level of β-arrestin2 mRNA and protein expression was decresed by 70% ±1.76% (P < 0.01) and 68.43% ± 2.88% (P < 0.01) as compared with the control group. Bcl-2 expression was also inhibited by 32.58% ±3.46% (P < 0.01) , while Bax expression was increased by 38.00% ±3.72% (P < 0.01). The apoptosis rate of HSC-T6 transfected with β-arrestin2 siRNA was 37.5% , which was significantly higher than control. Conclusion Inhibition of β-arrestin2 by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by promoting apoptosis of HSC.

AB - Aim: To investigate the effect of synthetic small interfering RNA (siRNA) targeting β-arrestin2 on the apoptosis of hepatic stellate cells (HSC) in vitro. Methods Synthetic siRNA targeting β-arrestin2 was transfected into HSC-T6 cells by lipofectamine package. Negative siRNA transfection and no transfection were used as negative and blank control, respectively. After incubation with siRNA, total RNA and protein of HSC-T6 cells were extracted. The expression of β-arrestin2 gene and protein was detected by reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting. HSC-T6 apoptosis was measured by flow cytometry. The expression of Bcl-2 and Bax were determined by Western blot. Results: After HSC-T6 cells were transfected with β-arrestin2 siRNA, the level of β-arrestin2 mRNA and protein expression was decresed by 70% ±1.76% (P < 0.01) and 68.43% ± 2.88% (P < 0.01) as compared with the control group. Bcl-2 expression was also inhibited by 32.58% ±3.46% (P < 0.01) , while Bax expression was increased by 38.00% ±3.72% (P < 0.01). The apoptosis rate of HSC-T6 transfected with β-arrestin2 siRNA was 37.5% , which was significantly higher than control. Conclusion Inhibition of β-arrestin2 by siRNA may have a potential effect on prevention and treatment of hepatic fibrosis by promoting apoptosis of HSC.

KW - Apoptosis

KW - Bax

KW - Bcl-2

KW - Hepatic fibrosis

KW - Hepatic stellate cells

KW - Small interfering RNA

KW - β-arrestin2

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