Small interfering RNA in drug metabolism and transport

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

RNA interference (RNAi) is a powerful technique that utilizes RNA molecules to specifically knock down the expression of targeted gene at posttranscriptional level. These small interfering RNAs (siRNAs) not only have broad application to basic biomedical research but may be developed as therapeutic agents. Drug-metabolizing enzymes (DMEs) and drug transporters (DTs) are molecular determinants of pharmacokinetic property of a drug. Transcriptional gene expression of DMEs and DTs is controlled by xenobiotic-sensing nuclear receptors (NRs). Because of complexity in studying the function of individual DMEs, DTs and Nits, siRNAs can be an excellent addition to chemical inhibitors and inhibitory antibodies in delineating their specific roles in drug metabolism and transport, gene regulation, and drug-drug interactions. RNAi may be employed to modulate DT expression to overcome multidrug resistance. Recent studies using RNAi to silence gene expression of specific DME, DT and NR, and the impact on drug metabolism and transport are discussed in this review. Concerns remain about the efficiency, specificity, and off-target effects when interpreting data obtained from RNAi studies. Furthermore, potential role for endogenous siRNAs, microRNA (miRNA) molecules, in controlling the posttranscriptional gene regulation of DMEs, DTs and NRs is discussed.

Original languageEnglish (US)
Pages (from-to)700-708
Number of pages9
JournalCurrent Drug Metabolism
Volume8
Issue number7
DOIs
StatePublished - Oct 2007
Externally publishedYes

Keywords

  • Drug metabolism and transport
  • Enzyme
  • Gene regulation
  • miRNA
  • Nuclear receptor
  • RNAi
  • siRNA
  • Transporter

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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