Small applied electric fields guide migration of hippocampal neurons

Li Yao, Lynne Shanley, Colin Mccaig, Min Zhao

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

Effectively directed neuron migration is critical for development and repair in the central nervous system (CNS). Endogenous electric fields (EFs) are widespread in developing and regenerating tissues and regulate a variety of cell behaviors including directed cell migration. Electrically-directed neuronal migration has not been tested previously and we show that an applied EF directs migration of hippocampal neurons toward the cathode at a field strength of 120 mV/mm, close to the physiological range. Reversal of the field polarity reversed the direction of neuron migration. Neuron migration from an explant also was directed by an applied EF. Mechanistically, EF-guided migration was transduced by activation of the second messenger molecules ROCK (Rho-associated protein kinase) and PI3 kinase (phosphoinositide-3 kinase) since their pharmacological inhibition decreased the directedness and speed of neuron migration. This work demonstrates that rat hippocampal neurons respond to applied EFs with directional migration and raises the possibility that EFs may be used as a cue to direct neuronal migration in novel strategies to repair the CNS.

Original languageEnglish (US)
Pages (from-to)527-535
Number of pages9
JournalJournal of Cellular Physiology
Volume216
Issue number2
DOIs
StatePublished - Aug 2008

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Fingerprint Dive into the research topics of 'Small applied electric fields guide migration of hippocampal neurons'. Together they form a unique fingerprint.

  • Cite this