Small applied electric fields guide migration of hippocampal neurons

Li Yao, Lynne Shanley, Colin Mccaig, Min Zhao

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Effectively directed neuron migration is critical for development and repair in the central nervous system (CNS). Endogenous electric fields (EFs) are widespread in developing and regenerating tissues and regulate a variety of cell behaviors including directed cell migration. Electrically-directed neuronal migration has not been tested previously and we show that an applied EF directs migration of hippocampal neurons toward the cathode at a field strength of 120 mV/mm, close to the physiological range. Reversal of the field polarity reversed the direction of neuron migration. Neuron migration from an explant also was directed by an applied EF. Mechanistically, EF-guided migration was transduced by activation of the second messenger molecules ROCK (Rho-associated protein kinase) and PI3 kinase (phosphoinositide-3 kinase) since their pharmacological inhibition decreased the directedness and speed of neuron migration. This work demonstrates that rat hippocampal neurons respond to applied EFs with directional migration and raises the possibility that EFs may be used as a cue to direct neuronal migration in novel strategies to repair the CNS.

Original languageEnglish (US)
Pages (from-to)527-535
Number of pages9
JournalJournal of Cellular Physiology
Volume216
Issue number2
DOIs
StatePublished - Aug 2008

Fingerprint

Neurons
Electric fields
Neurology
Repair
Central Nervous System
rho-Associated Kinases
1-Phosphatidylinositol 4-Kinase
Second Messenger Systems
Phosphatidylinositols
Phosphatidylinositol 3-Kinases
Protein Kinases
Cell Movement
Cues
Rats
Electrodes
Cathodes
Phosphotransferases
Chemical activation
Pharmacology
Tissue

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Small applied electric fields guide migration of hippocampal neurons. / Yao, Li; Shanley, Lynne; Mccaig, Colin; Zhao, Min.

In: Journal of Cellular Physiology, Vol. 216, No. 2, 08.2008, p. 527-535.

Research output: Contribution to journalArticle

Yao, Li ; Shanley, Lynne ; Mccaig, Colin ; Zhao, Min. / Small applied electric fields guide migration of hippocampal neurons. In: Journal of Cellular Physiology. 2008 ; Vol. 216, No. 2. pp. 527-535.
@article{e219e9e1832e4190ad9d3d8a6dab3e75,
title = "Small applied electric fields guide migration of hippocampal neurons",
abstract = "Effectively directed neuron migration is critical for development and repair in the central nervous system (CNS). Endogenous electric fields (EFs) are widespread in developing and regenerating tissues and regulate a variety of cell behaviors including directed cell migration. Electrically-directed neuronal migration has not been tested previously and we show that an applied EF directs migration of hippocampal neurons toward the cathode at a field strength of 120 mV/mm, close to the physiological range. Reversal of the field polarity reversed the direction of neuron migration. Neuron migration from an explant also was directed by an applied EF. Mechanistically, EF-guided migration was transduced by activation of the second messenger molecules ROCK (Rho-associated protein kinase) and PI3 kinase (phosphoinositide-3 kinase) since their pharmacological inhibition decreased the directedness and speed of neuron migration. This work demonstrates that rat hippocampal neurons respond to applied EFs with directional migration and raises the possibility that EFs may be used as a cue to direct neuronal migration in novel strategies to repair the CNS.",
author = "Li Yao and Lynne Shanley and Colin Mccaig and Min Zhao",
year = "2008",
month = "8",
doi = "10.1002/jcp.21431",
language = "English (US)",
volume = "216",
pages = "527--535",
journal = "Journal of Cellular Physiology",
issn = "0021-9541",
publisher = "Wiley-Liss Inc.",
number = "2",

}

TY - JOUR

T1 - Small applied electric fields guide migration of hippocampal neurons

AU - Yao, Li

AU - Shanley, Lynne

AU - Mccaig, Colin

AU - Zhao, Min

PY - 2008/8

Y1 - 2008/8

N2 - Effectively directed neuron migration is critical for development and repair in the central nervous system (CNS). Endogenous electric fields (EFs) are widespread in developing and regenerating tissues and regulate a variety of cell behaviors including directed cell migration. Electrically-directed neuronal migration has not been tested previously and we show that an applied EF directs migration of hippocampal neurons toward the cathode at a field strength of 120 mV/mm, close to the physiological range. Reversal of the field polarity reversed the direction of neuron migration. Neuron migration from an explant also was directed by an applied EF. Mechanistically, EF-guided migration was transduced by activation of the second messenger molecules ROCK (Rho-associated protein kinase) and PI3 kinase (phosphoinositide-3 kinase) since their pharmacological inhibition decreased the directedness and speed of neuron migration. This work demonstrates that rat hippocampal neurons respond to applied EFs with directional migration and raises the possibility that EFs may be used as a cue to direct neuronal migration in novel strategies to repair the CNS.

AB - Effectively directed neuron migration is critical for development and repair in the central nervous system (CNS). Endogenous electric fields (EFs) are widespread in developing and regenerating tissues and regulate a variety of cell behaviors including directed cell migration. Electrically-directed neuronal migration has not been tested previously and we show that an applied EF directs migration of hippocampal neurons toward the cathode at a field strength of 120 mV/mm, close to the physiological range. Reversal of the field polarity reversed the direction of neuron migration. Neuron migration from an explant also was directed by an applied EF. Mechanistically, EF-guided migration was transduced by activation of the second messenger molecules ROCK (Rho-associated protein kinase) and PI3 kinase (phosphoinositide-3 kinase) since their pharmacological inhibition decreased the directedness and speed of neuron migration. This work demonstrates that rat hippocampal neurons respond to applied EFs with directional migration and raises the possibility that EFs may be used as a cue to direct neuronal migration in novel strategies to repair the CNS.

UR - http://www.scopus.com/inward/record.url?scp=46049120443&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=46049120443&partnerID=8YFLogxK

U2 - 10.1002/jcp.21431

DO - 10.1002/jcp.21431

M3 - Article

C2 - 18393356

AN - SCOPUS:46049120443

VL - 216

SP - 527

EP - 535

JO - Journal of Cellular Physiology

JF - Journal of Cellular Physiology

SN - 0021-9541

IS - 2

ER -