Abstract
We have previously demonstrated that p100H mutant mice, which lack a functional Sox6 gene, exhibit skeletal and cardiac muscle degeneration and develop cardiac conduction abnormalities soon after birth. To understand the role of Sox6 in skeletal muscle development, we identified muscle-specific genes differentially expressed between wild-type and p100H mutant skeletal muscles and investigated their temporal expression in the mutant muscle. We found that, in the mutant skeletal muscle, slow fiber and cardiac isoform genes are expressed at significantly higher levels, whereas fast fiber isoform genes are expressed at significantly lower levels than wild-type. Onset of this aberrant fiber type-specific gene expression in the mutant coincides with the beginning of the secondary myotube formation, at embryonic day 15-16 in mice. Together with our earlier report, demonstrating early postnatal muscle defects in the Sox6 null-p100H mutant, the present results suggest that Sox6 likely plays an important role in muscle development.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 301-311 |
| Number of pages | 11 |
| Journal | Developmental Dynamics |
| Volume | 234 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 2005 |
Fingerprint
Keywords
- Skeletal muscle development
- Slow and fast skeletal isoform genes
- Sox6
ASJC Scopus subject areas
- Developmental Biology
- Cell Biology
Cite this
Slow and fast fiber isoform gene expression is systematically altered in skeletal muscle of the Sox6 mutant, p100H . / Hagiwara, Nobuko; Ma, Betty; Ly, Alice.
In: Developmental Dynamics, Vol. 234, No. 2, 10.2005, p. 301-311.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Slow and fast fiber isoform gene expression is systematically altered in skeletal muscle of the Sox6 mutant, p100H
AU - Hagiwara, Nobuko
AU - Ma, Betty
AU - Ly, Alice
PY - 2005/10
Y1 - 2005/10
N2 - We have previously demonstrated that p100H mutant mice, which lack a functional Sox6 gene, exhibit skeletal and cardiac muscle degeneration and develop cardiac conduction abnormalities soon after birth. To understand the role of Sox6 in skeletal muscle development, we identified muscle-specific genes differentially expressed between wild-type and p100H mutant skeletal muscles and investigated their temporal expression in the mutant muscle. We found that, in the mutant skeletal muscle, slow fiber and cardiac isoform genes are expressed at significantly higher levels, whereas fast fiber isoform genes are expressed at significantly lower levels than wild-type. Onset of this aberrant fiber type-specific gene expression in the mutant coincides with the beginning of the secondary myotube formation, at embryonic day 15-16 in mice. Together with our earlier report, demonstrating early postnatal muscle defects in the Sox6 null-p100H mutant, the present results suggest that Sox6 likely plays an important role in muscle development.
AB - We have previously demonstrated that p100H mutant mice, which lack a functional Sox6 gene, exhibit skeletal and cardiac muscle degeneration and develop cardiac conduction abnormalities soon after birth. To understand the role of Sox6 in skeletal muscle development, we identified muscle-specific genes differentially expressed between wild-type and p100H mutant skeletal muscles and investigated their temporal expression in the mutant muscle. We found that, in the mutant skeletal muscle, slow fiber and cardiac isoform genes are expressed at significantly higher levels, whereas fast fiber isoform genes are expressed at significantly lower levels than wild-type. Onset of this aberrant fiber type-specific gene expression in the mutant coincides with the beginning of the secondary myotube formation, at embryonic day 15-16 in mice. Together with our earlier report, demonstrating early postnatal muscle defects in the Sox6 null-p100H mutant, the present results suggest that Sox6 likely plays an important role in muscle development.
KW - Skeletal muscle development
KW - Slow and fast skeletal isoform genes
KW - Sox6
UR - http://www.scopus.com/inward/record.url?scp=25444510604&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=25444510604&partnerID=8YFLogxK
U2 - 10.1002/dvdy.20535
DO - 10.1002/dvdy.20535
M3 - Article
C2 - 16124007
AN - SCOPUS:25444510604
VL - 234
SP - 301
EP - 311
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 2
ER -