Aquaporin-4 (AQP4) is a water conducting membrane integral protein channel which is widely expressed in the astrocyte system of the brain. During the development of the AQP4 positron emission tomography (PET) imaging agent [11C]TGN-020 (N-(1,3,4-thiadiazol-2-yl)pyridine-3-[11C]-carboxamide), significant radioligand uptake was observed in the skull, where there was no known distribution of any aquaporin family proteins. Herein we confirmed via a newly developed method for bone-tissue immunohistology, a hitherto unrecognized distribution of AQP4, and not AQP1, in the skull. Other bony structures, by contrast, showed virtually no uptake of [11C]TGN-020, and likewise, do not express either AQP4 or AQP1. Immunohistological analysis demonstrated that the AQP4 expression in the skull is restricted to the diploë. Consequently, we suspect AQP4 plays a pivotal role in the formation and maintenance of yellow marrow and the diploë. However, elucidating the exact nature of that role will require further studies. Anatomy; Pathology; Medical imaging; Nuclear medicine; Radiology; Tissue culture; Bone; Aquaporin 4 (AQP4); Skull; Positron emission tomography(PET); N-(1,3,4-thiadiazol-2-yl) pyridine-3-[11C]-carboxamide ([11C]TGN-020); Immunohistology.
- Aquaporin 4 (AQP4)
- Medical imaging
- N-(1,3,4-thiadiazol-2-yl) pyridine-3-[11C]-carboxamide ([11C]TGN-020)
- Nuclear medicine
- Positron emission tomography(PET)
- Tissue culture
ASJC Scopus subject areas