Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers

S. T. Boyce, David G Greenhalgh, R. J. Kagan, T. Housinger, J. M. Sorrell, C. P. Childress, M. Rieman, G. D. Warden

Research output: Contribution to journalArticle

88 Scopus citations

Abstract

Closure of large skin wounds (i.e., burns, congenital giant nevus, reconstruction of traumatic injury) with split-thickness skin grafts requires extensive harvesting of autologous skin. Composite grafts consisting of collagen-glycosaminoglycan (GAG) substrates populated with cultured dermal fibroblasts and epidermal keratinocytes were tested in a pilot study on full- thickness burn wounds of three patients as an alternative to split-thickness skin. Light microscopy and transmission electron microscopy showed regeneration of epidermal and dermal tissue by 2 weeks, with degradation of the collagen-GAG implant associated with low numbers of leukocytes, and deposition of new collagen by fibroblasts. Complete basement membrane, including anchoring fibrils and anchoring plaques, is formed by 2 weeks, is mature by 3 months, and accounts for the absence of blistering of healed epidermis. All skin antigens tested (involucrin, filaggrin, laminin, collagens IV and VII, fibronectin, and chondroitin-sulfate) were expressed by 16 days after grafting. This cultured skin analogue provides an experimental alternative to split-thickness skin graft that develops histiotypic markers of skin anatomy and antigen expression after wound closure.

Original languageEnglish (US)
Pages (from-to)632-641
Number of pages10
JournalPlastic and Reconstructive Surgery
Volume91
Issue number4
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Surgery

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    Boyce, S. T., Greenhalgh, D. G., Kagan, R. J., Housinger, T., Sorrell, J. M., Childress, C. P., Rieman, M., & Warden, G. D. (1993). Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers. Plastic and Reconstructive Surgery, 91(4), 632-641.