Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers

S. T. Boyce, David G Greenhalgh, R. J. Kagan, T. Housinger, J. M. Sorrell, C. P. Childress, M. Rieman, G. D. Warden

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Closure of large skin wounds (i.e., burns, congenital giant nevus, reconstruction of traumatic injury) with split-thickness skin grafts requires extensive harvesting of autologous skin. Composite grafts consisting of collagen-glycosaminoglycan (GAG) substrates populated with cultured dermal fibroblasts and epidermal keratinocytes were tested in a pilot study on full- thickness burn wounds of three patients as an alternative to split-thickness skin. Light microscopy and transmission electron microscopy showed regeneration of epidermal and dermal tissue by 2 weeks, with degradation of the collagen-GAG implant associated with low numbers of leukocytes, and deposition of new collagen by fibroblasts. Complete basement membrane, including anchoring fibrils and anchoring plaques, is formed by 2 weeks, is mature by 3 months, and accounts for the absence of blistering of healed epidermis. All skin antigens tested (involucrin, filaggrin, laminin, collagens IV and VII, fibronectin, and chondroitin-sulfate) were expressed by 16 days after grafting. This cultured skin analogue provides an experimental alternative to split-thickness skin graft that develops histiotypic markers of skin anatomy and antigen expression after wound closure.

Original languageEnglish (US)
Pages (from-to)632-641
Number of pages10
JournalPlastic and Reconstructive Surgery
Volume91
Issue number4
StatePublished - 1993
Externally publishedYes

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Biopolymers
Cultured Cells
Anatomy
Transplants
Antigens
Skin
Wounds and Injuries
Collagen
Glycosaminoglycans
Fibroblasts
Chondroitin Sulfates
Nevus
Laminin
Transmission Electron Microscopy
Burns
Leukocyte Count
Keratinocytes
Fibronectins
Basement Membrane
Epidermis

ASJC Scopus subject areas

  • Surgery

Cite this

Boyce, S. T., Greenhalgh, D. G., Kagan, R. J., Housinger, T., Sorrell, J. M., Childress, C. P., ... Warden, G. D. (1993). Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers. Plastic and Reconstructive Surgery, 91(4), 632-641.

Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers. / Boyce, S. T.; Greenhalgh, David G; Kagan, R. J.; Housinger, T.; Sorrell, J. M.; Childress, C. P.; Rieman, M.; Warden, G. D.

In: Plastic and Reconstructive Surgery, Vol. 91, No. 4, 1993, p. 632-641.

Research output: Contribution to journalArticle

Boyce, ST, Greenhalgh, DG, Kagan, RJ, Housinger, T, Sorrell, JM, Childress, CP, Rieman, M & Warden, GD 1993, 'Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers', Plastic and Reconstructive Surgery, vol. 91, no. 4, pp. 632-641.
Boyce, S. T. ; Greenhalgh, David G ; Kagan, R. J. ; Housinger, T. ; Sorrell, J. M. ; Childress, C. P. ; Rieman, M. ; Warden, G. D. / Skin anatomy and antigen expression after burn wound closure with composite grafts of cultured skin cells and biopolymers. In: Plastic and Reconstructive Surgery. 1993 ; Vol. 91, No. 4. pp. 632-641.
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