Skeletal muscle hemoglobin content measured by near infrared spectroscopy during oscillatory venous pressure variation

Laura Marcu, Jean Michel L. Maarek, Atul Bembi, Sandra HowelL

Research output: Contribution to journalConference articlepeer-review

3 Scopus citations


Changes in oxidized (HbO), reduced (Hbr), and total hemoglobin (Hbt) contents were monitored by near infrared spectroscopy in huimrn forearm skeletal muscle during oscillatory variations of the effective venous pressure. Laser diode pulses (wavelength, 775, 810, 865, 904; duration, 60 ns) were directed to the muscle by means of an optic fiber bundle and detected with a sensing fiber positioned at 1.5 cm from the emitting bundle. Sinusoidal pressure waves (frequency, 1 and 2 cycles/mm; amplitude, 10-15 mm Hg) generated by a piston pump were transmitted to a sphygmomanometer cuffplaced on the arm, the mean cuffpressure (Pc) being 20, 40 and 60 mm Hg. Variations of HbO, Hbr and Hbt were computed from the optical signals and processed by Fourier analysis to characterize their amplitude and phase relative to the cuff man pressure oscillations (Posc). Oscillations of HbO, Hbr, Hbt were observed for all mean cuff pressures, the amplitude of the variations being decreased with increasing Pc. For Pc=20 mm Hg, the oscillations of HbO and Hbt were nearly in phase with Posc whereas the oscillation ofHbr were out ofphase with HbO and Posc. Increasing Pc resulted in an increase of the phase difference between HbO and Posc, Hbr remaining out of phase with Posc. These trends could be predicted with a lumped model of the forearm vasculature, suggesting that the technique could be used to assess mechanical characteristics of vascular beds.

Original languageEnglish (US)
Pages (from-to)218-224
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
StatePublished - Apr 12 1995
Externally publishedYes
EventAdvances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II 1995 - San Jose, United States
Duration: Feb 1 1995Feb 28 1995


  • Forearm Skeletal Muscle
  • Fourier Analysis
  • Hemoglobin
  • Near Infrared Spectroscopy
  • Venous Pressure

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


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