Abstract
Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. Two domains within its replication-defective genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. An efficient transfection system for generating infectious, transforming virus from molecular clones of AEV and RAV-1 (helper virus) was combined with the techniques of site-specific mutagenesis to investigate the contribution of erb-B to the two forms of oncogenesis induced by AEV. Deletion and frameshift mutations were constructed in the erb-B locus of cloned AEV DNA in vitro. Infectious retroviruses harboring these mutations were recovered and their ability to transform fibroblasts in vitro or induce erythroleukemia in vivo was assessed. The presence of mutant viral genomes in chick embryo fibroblasts or erythroblasts of infected birds was confirmed by suitable biochemical analyses. Expression of viral genes in cells infected with AEV mutants was examined by immunoprecipitation with antisera to erb-A and erb-B proteins. It was found that the product of erb-B is necessary for transformation of fibroblasts and induction of erythroblastosis by AEV, although a small portion of this protein at the carboxy terminus is dispensable.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 155-178 |
| Number of pages | 24 |
| Journal | Virology |
| Volume | 130 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 15 1983 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Virology
- Infectious Diseases
Cite this
Site-specific mutagenesis of avian erythroblastosis virus : erb-B is required for oncogenicity. / Sealy, Linda; Privalsky, Martin L.; Moscovici, Giovanella; Moscovici, Carlo; Bishop, J. Michael.
In: Virology, Vol. 130, No. 1, 15.10.1983, p. 155-178.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Site-specific mutagenesis of avian erythroblastosis virus
T2 - erb-B is required for oncogenicity
AU - Sealy, Linda
AU - Privalsky, Martin L.
AU - Moscovici, Giovanella
AU - Moscovici, Carlo
AU - Bishop, J. Michael
PY - 1983/10/15
Y1 - 1983/10/15
N2 - Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. Two domains within its replication-defective genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. An efficient transfection system for generating infectious, transforming virus from molecular clones of AEV and RAV-1 (helper virus) was combined with the techniques of site-specific mutagenesis to investigate the contribution of erb-B to the two forms of oncogenesis induced by AEV. Deletion and frameshift mutations were constructed in the erb-B locus of cloned AEV DNA in vitro. Infectious retroviruses harboring these mutations were recovered and their ability to transform fibroblasts in vitro or induce erythroleukemia in vivo was assessed. The presence of mutant viral genomes in chick embryo fibroblasts or erythroblasts of infected birds was confirmed by suitable biochemical analyses. Expression of viral genes in cells infected with AEV mutants was examined by immunoprecipitation with antisera to erb-A and erb-B proteins. It was found that the product of erb-B is necessary for transformation of fibroblasts and induction of erythroblastosis by AEV, although a small portion of this protein at the carboxy terminus is dispensable.
AB - Avian erythroblastosis virus (AEV) induces both erythroblastosis and fibrosarcomas in susceptible birds. Two domains within its replication-defective genome, erb-A and erb-B, have been implicated in AEV-mediated oncogenesis. An efficient transfection system for generating infectious, transforming virus from molecular clones of AEV and RAV-1 (helper virus) was combined with the techniques of site-specific mutagenesis to investigate the contribution of erb-B to the two forms of oncogenesis induced by AEV. Deletion and frameshift mutations were constructed in the erb-B locus of cloned AEV DNA in vitro. Infectious retroviruses harboring these mutations were recovered and their ability to transform fibroblasts in vitro or induce erythroleukemia in vivo was assessed. The presence of mutant viral genomes in chick embryo fibroblasts or erythroblasts of infected birds was confirmed by suitable biochemical analyses. Expression of viral genes in cells infected with AEV mutants was examined by immunoprecipitation with antisera to erb-A and erb-B proteins. It was found that the product of erb-B is necessary for transformation of fibroblasts and induction of erythroblastosis by AEV, although a small portion of this protein at the carboxy terminus is dispensable.
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U2 - 10.1016/0042-6822(83)90125-3
DO - 10.1016/0042-6822(83)90125-3
M3 - Article
C2 - 6195816
AN - SCOPUS:0021064999
VL - 130
SP - 155
EP - 178
JO - Virology
JF - Virology
SN - 0042-6822
IS - 1
ER -