Site-specific initiation of transcription by RNA polymerase II

R. Kollmar, P. J. Farnham

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

RNA polymerase II initiates transcription at specific DNA sequences. Studies using sequence analysis and molecular genetics suggest a simple and universal model of start-site selection by RNA polymerase II. Two consensus sequences occur at fixed positions in promoters from higher eukaryotes and their viruses: the TATA box around -30 and the initiator at the start site of transcription. Both consensus sequences function as positioning elements that control site-specific initiation. As a first step during initiation, the basal transcription factor TFIID binds to the TATA box; regulatory transcription factors can tether TFIID to promoters without a consensus TATA box. TFIID then directs the assembly of other basal transcription factors and RNA polymerase II into a preinitiation complex. Finally, RNA polymerase II searches for the best match to the initiator consensus about 30 base pairs downstream of the TATA box to select the exact start site. The transcriptional activity of a start-site sequence generally correlates with its similarity to the initiator consensus, suggesting that there is only one type of initiator.

Original languageEnglish (US)
Pages (from-to)127-139
Number of pages13
JournalProceedings of the Society for Experimental Biology and Medicine
Volume203
Issue number2
StatePublished - 1993
Externally publishedYes

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TATA Box
RNA Polymerase II
Transcription Initiation Site
Transcription Factor TFIID
Transcription
Consensus Sequence
Transcription Factors
Site selection
DNA sequences
Eukaryota
Viruses
Base Pairing
Sequence Analysis
Molecular Biology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Site-specific initiation of transcription by RNA polymerase II. / Kollmar, R.; Farnham, P. J.

In: Proceedings of the Society for Experimental Biology and Medicine, Vol. 203, No. 2, 1993, p. 127-139.

Research output: Contribution to journalArticle

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