Site-directed genome modification: Derivatives of DNA-modifying enzymes as targeting tools

Craig J. Coates, Joseph M. Kaminski, James B. Summers, David Segal, Andrew D. Miller, Andreas F. Kolb

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The modification of mammalian genomes is an important goal in gene therapy and animal transgenesis. To generate stable genetic and biochemical changes, the therapeutic genes or transgenes need to be incorporated into the host genome. Ideally, the integration of the foreign gene should occur at sites that ensure their continual expression in the absence of any unwanted side effects on cellular metabolism. In this article, we discuss the opportunities provided by natural DNA-modifying enzymes, such as transposases, recombinases and integrases, to mediate the stable integration of foreign genes into host genomes. In addition, we discuss the approaches that have been taken to improve the efficiency and to modify the site-specificity of these enzymes.

Original languageEnglish (US)
Pages (from-to)407-419
Number of pages13
JournalTrends in Biotechnology
Volume23
Issue number8
DOIs
StatePublished - Aug 2005
Externally publishedYes

ASJC Scopus subject areas

  • Bioengineering

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