Sister chromatid exchange as an indicator of mutagenesis

A. V. Carrano, L. H. Thompson, P. A. Lindl, J. L. Minkler

Research output: Contribution to journalArticle

429 Citations (Scopus)

Abstract

SISTER chromatid exchange (SCE), that is, the reciprocal interchange of DNA between chromatids, is easily visualised in metaphase chromosomes1,2 and has been applied to study chromosome structure3,4, chromosome damage5, and instability and DNA repair deficiency syndromes 6-9. Since SCEs can be induced by subtoxic doses of carcinogens and mutagens5,10-13, their analysis offers the possibility of a rapid, sensitive and quantitative assay for genetic damage. We have begun to examine the relation between SCEs and mutations in Chinese hamster ovary (CHO) cells by quantifying the induction of SCEs in parallel with the induction of mutations producing 8-azaguanine resistance, that is, mutations predominately at the hypoxanthine phosphoribosyltransferase, hprt, locus. Since the conversion of a chemically induced DNA lesion to a SCE or mutation may depend on the nature of that lesion, we tested four chemicals that differ in their interaction with DNA - ethyl methanesulphonate (EMS; O6: N7 guanyl alkylation ratio14 of 0.03), N-ethyl-N-nitrosourea (ENU; O6: N 7 guanyl alkylation ratio15 of about 0.35), the crosslinking agent mitomycin C (MMC)16 and the intercalator proflavine sulphate (PRO)17. Our results indicate a linear relation between induced SCEs and mutations. The relative efficiency, however, is different for each chemical.

Original languageEnglish (US)
Pages (from-to)551-553
Number of pages3
JournalNature
Volume271
Issue number5645
DOIs
StatePublished - 1978
Externally publishedYes

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Sister Chromatid Exchange
Mutagenesis
Chromatids
Mutation
Alkylation
DNA
Azaguanine
Proflavine
DNA Repair-Deficiency Disorders
Intercalating Agents
Ethyl Methanesulfonate
Ethylnitrosourea
Hypoxanthine Phosphoribosyltransferase
Chromosomal Instability
Chromosomes, Human, Pair 4
Mitomycin
Metaphase
Cricetulus
Carcinogens
Sulfates

ASJC Scopus subject areas

  • General

Cite this

Carrano, A. V., Thompson, L. H., Lindl, P. A., & Minkler, J. L. (1978). Sister chromatid exchange as an indicator of mutagenesis. Nature, 271(5645), 551-553. https://doi.org/10.1038/271551a0

Sister chromatid exchange as an indicator of mutagenesis. / Carrano, A. V.; Thompson, L. H.; Lindl, P. A.; Minkler, J. L.

In: Nature, Vol. 271, No. 5645, 1978, p. 551-553.

Research output: Contribution to journalArticle

Carrano, AV, Thompson, LH, Lindl, PA & Minkler, JL 1978, 'Sister chromatid exchange as an indicator of mutagenesis', Nature, vol. 271, no. 5645, pp. 551-553. https://doi.org/10.1038/271551a0
Carrano AV, Thompson LH, Lindl PA, Minkler JL. Sister chromatid exchange as an indicator of mutagenesis. Nature. 1978;271(5645):551-553. https://doi.org/10.1038/271551a0
Carrano, A. V. ; Thompson, L. H. ; Lindl, P. A. ; Minkler, J. L. / Sister chromatid exchange as an indicator of mutagenesis. In: Nature. 1978 ; Vol. 271, No. 5645. pp. 551-553.
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