siRNA-directed inhibition of HIV-1 infection

Carl D. Novina, Michael F. Murray, Derek M. Dykxhoorn, Paul J. Beresford, Jonathan Riess, Sang Kyung Lee, Ronald G. Collman, Judy Lieberman, Premlata Shankar, Phillip A. Sharp

Research output: Contribution to journalArticle

710 Scopus citations

Abstract

RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.

Original languageEnglish (US)
Pages (from-to)681-686
Number of pages6
JournalNature Medicine
Volume8
Issue number7
DOIs
StatePublished - 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

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  • Cite this

    Novina, C. D., Murray, M. F., Dykxhoorn, D. M., Beresford, P. J., Riess, J., Lee, S. K., Collman, R. G., Lieberman, J., Shankar, P., & Sharp, P. A. (2002). siRNA-directed inhibition of HIV-1 infection. Nature Medicine, 8(7), 681-686. https://doi.org/10.1038/nm725