siRNA-directed inhibition of HIV-1 infection

Carl D. Novina, Michael F. Murray, Derek M. Dykxhoorn, Paul J. Beresford, Jonathan Riess, Sang Kyung Lee, Ronald G. Collman, Judy Lieberman, Premlata Shankar, Phillip A. Sharp

Research output: Contribution to journalArticle

710 Citations (Scopus)

Abstract

RNA interference silences gene expression through short interfering 21-23-mer double-strand RNA segments that guide mRNA degradation in a sequence-specific fashion. Here we report that siRNAs inhibit virus production by targeting the mRNAs for either the HIV-1 cellular receptor CD4, the viral structural Gag protein or green fluorescence protein substituted for the Nef regulatory protein. siRNAs effectively inhibit pre- and/or post-integration infection events in the HIV-1 life cycle. Thus, siRNAs may have potential for therapeutic intervention in HIV-1 and other viral infections.

Original languageEnglish (US)
Pages (from-to)681-686
Number of pages6
JournalNature Medicine
Volume8
Issue number7
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Small Interfering RNA
HIV Infections
HIV-1
RNA
gag Gene Products
CD4 Antigens
Messenger RNA
Guide RNA
nef Gene Products
Viruses
Gene expression
Viral Structural Proteins
Life cycle
Proteins
Fluorescence
RNA Stability
Virus Diseases
RNA Interference
Life Cycle Stages
Degradation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Novina, C. D., Murray, M. F., Dykxhoorn, D. M., Beresford, P. J., Riess, J., Lee, S. K., ... Sharp, P. A. (2002). siRNA-directed inhibition of HIV-1 infection. Nature Medicine, 8(7), 681-686. https://doi.org/10.1038/nm725

siRNA-directed inhibition of HIV-1 infection. / Novina, Carl D.; Murray, Michael F.; Dykxhoorn, Derek M.; Beresford, Paul J.; Riess, Jonathan; Lee, Sang Kyung; Collman, Ronald G.; Lieberman, Judy; Shankar, Premlata; Sharp, Phillip A.

In: Nature Medicine, Vol. 8, No. 7, 2002, p. 681-686.

Research output: Contribution to journalArticle

Novina, CD, Murray, MF, Dykxhoorn, DM, Beresford, PJ, Riess, J, Lee, SK, Collman, RG, Lieberman, J, Shankar, P & Sharp, PA 2002, 'siRNA-directed inhibition of HIV-1 infection', Nature Medicine, vol. 8, no. 7, pp. 681-686. https://doi.org/10.1038/nm725
Novina CD, Murray MF, Dykxhoorn DM, Beresford PJ, Riess J, Lee SK et al. siRNA-directed inhibition of HIV-1 infection. Nature Medicine. 2002;8(7):681-686. https://doi.org/10.1038/nm725
Novina, Carl D. ; Murray, Michael F. ; Dykxhoorn, Derek M. ; Beresford, Paul J. ; Riess, Jonathan ; Lee, Sang Kyung ; Collman, Ronald G. ; Lieberman, Judy ; Shankar, Premlata ; Sharp, Phillip A. / siRNA-directed inhibition of HIV-1 infection. In: Nature Medicine. 2002 ; Vol. 8, No. 7. pp. 681-686.
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