SipA, SopA, SopB, SopD, and SopE2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells

Manuela Raffatellu, R. Paul Wilson, Daniela Chessa, Helene Andrews-Polymenis, Quynh T. Tran, Sara Lawhon, Sangeeta Khare, L. Garry Adams, Andreas J Baumler

Research output: Contribution to journalArticle

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Abstract

The centisome 63 type III secretion system (T3SS-1) encoded by Salmonella pathogenicity island 1 (SPI1) mediates invasion of epithelial cells by Salmonella enterica serotype Typhimurium. Characterization of mutants lacking individual genes has revealed that T3SS-1 secreted proteins (effectors) SopE2 and SopB are required for invasion while the SipA protein accelerates entry into cells. Here we have revisited the question of which T3SS-1 effectors contribute to the invasion of epithelial cells by complementing a strain lacking all of the effector genes that are required to cause diarrhea in a calf (a sipA sopABDE2 mutant). Introduction of either the cloned sipA, the cloned sopB, or the cloned sopE2 gene increased the invasiveness of the sipA sopABDE2 mutant for nonpolarized HT-29 cells. However, a contribution of sopA or sopD to invasion was not apparent when invasion assays were performed with the nonpolarized colon carcinoma cell lines T84 and HT-29. In contrast, introduction of either the sopA, the sopB, the sopD, or the sopE2 gene increased the invasiveness of the sipA sopABDE2 mutant for polarized T84 cells. Furthermore, introduction of a plasmid carrying sipA and sopB increased the invasiveness of the sipA sopABDE2 mutant for polarized T84 cells significantly compared to the introduction of plasmids carrying only sipA or sopB. We conclude that SipA, SopA, SopB, SopD, and SopE2 contribute to S. enterica serotype Typhimurium invasion of epithelial cells in vitro.

Original languageEnglish (US)
Pages (from-to)146-154
Number of pages9
JournalInfection and Immunity
Volume73
Issue number1
DOIs
StatePublished - Jan 2005
Externally publishedYes

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Salmonella enterica
Epithelial Cells
Genes
Plasmids
Genomic Islands
HT29 Cells
Salmonella
Diarrhea
Colon
Proteins
Carcinoma
Cell Line
Serogroup

ASJC Scopus subject areas

  • Immunology

Cite this

SipA, SopA, SopB, SopD, and SopE2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells. / Raffatellu, Manuela; Wilson, R. Paul; Chessa, Daniela; Andrews-Polymenis, Helene; Tran, Quynh T.; Lawhon, Sara; Khare, Sangeeta; Adams, L. Garry; Baumler, Andreas J.

In: Infection and Immunity, Vol. 73, No. 1, 01.2005, p. 146-154.

Research output: Contribution to journalArticle

Raffatellu, M, Wilson, RP, Chessa, D, Andrews-Polymenis, H, Tran, QT, Lawhon, S, Khare, S, Adams, LG & Baumler, AJ 2005, 'SipA, SopA, SopB, SopD, and SopE2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells', Infection and Immunity, vol. 73, no. 1, pp. 146-154. https://doi.org/10.1128/IAI.73.1.146-154.2005
Raffatellu, Manuela ; Wilson, R. Paul ; Chessa, Daniela ; Andrews-Polymenis, Helene ; Tran, Quynh T. ; Lawhon, Sara ; Khare, Sangeeta ; Adams, L. Garry ; Baumler, Andreas J. / SipA, SopA, SopB, SopD, and SopE2 contribute to Salmonella enterica serotype typhimurium invasion of epithelial cells. In: Infection and Immunity. 2005 ; Vol. 73, No. 1. pp. 146-154.
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AU - Wilson, R. Paul

AU - Chessa, Daniela

AU - Andrews-Polymenis, Helene

AU - Tran, Quynh T.

AU - Lawhon, Sara

AU - Khare, Sangeeta

AU - Adams, L. Garry

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