Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: Integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1

K. W. Hunter, M. L. Watson, J. Rochelle, S. Ontiveros, D. Munroe, Michael F Seldin, D. E. Housman

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Interspersed repetitive sequence (IRS) PCR and repetitive element-to- bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15, 2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high- density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross.

Original languageEnglish (US)
Pages (from-to)510-519
Number of pages10
JournalGenomics
Volume18
Issue number3
StatePublished - 1993
Externally publishedYes

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ASJC Scopus subject areas

  • Genetics

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