Abstract
Interspersed repetitive sequence (IRS) PCR and repetitive element-to- bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15, 2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high- density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 510-519 |
| Number of pages | 10 |
| Journal | Genomics |
| Volume | 18 |
| Issue number | 3 |
| State | Published - 1993 |
| Externally published | Yes |
ASJC Scopus subject areas
- Genetics
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Hunter, K. W., Watson, M. L., Rochelle, J., Ontiveros, S., Munroe, D., Seldin, M. F., & Housman, D. E. (1993). Single-strand conformational polymorphism (SSCP) mapping of the mouse genome: Integration of the SSCP, microsatellite, and gene maps of mouse chromosome 1. Genomics, 18(3), 510-519.