Interspersed repetitive sequence (IRS) PCR and repetitive element-to- bubble (IRS-bubble) PCR have been utilized to rapidly generate large numbers of mouse-specific, chromosome 1-enriched STSs from mouse-hamster somatic cell hybrids. Single-strand conformational polymorphism (SSCP) has been used to localize 39 new repetitive element-linked STSs to the mouse map: 22 to Chr 1, 10 to Chr. 15, 2 each to Chrs 12 and 14, and three to Chr 7. In addition, we have integrated the SSCP, single-strand length polymorphism, and restriction fragment length polymorphism maps of mouse Chr. 1, resulting in a high- density map of the chromosome, containing over 100 loci, all typed on a single interspecific backcross.
|Original language||English (US)|
|Number of pages||10|
|State||Published - 1993|
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