Abstract
We demonstrate for the first time an adaptive optics (AO) spectral OCT retina camera that acquires with unprecedented 3D resolution (2.9 μm lateral; 5.5 μm axial) single shot B-scans of the living human retina. The camera centers on a Michelson interferometer that consists of a superluminescent diode for line illuminating the subject's retinal; voice coil translator for controlling the optical path length of the reference channel; and an imaging spectrometer that is cascaded with a 12-bit area CCD array. The imaging spectrometer was designed with negligible off-axis aberrations and was constructed from stock optical components. AO was integrated into the detector channel of the interferometer and dynamically compensated for most of the ocular aberration across a 6 mm pupil. Short bursts of B-scans, with 100 A-scans each, were successfully acquired at 1 msec intervals. Camera sensitivity was found sufficient to detect reflections from all major retinal layers. Individual outer segments of photoreceptors at different retinal eccentricities were observed in vivo. Periodicity of the outer segments matched cone spacing as measured from AO flood illuminated images of the same patches of retina.
| Original language | English (US) |
|---|---|
| Article number | 95 |
| Pages (from-to) | 548-555 |
| Number of pages | 8 |
| Journal | Progress in Biomedical Optics and Imaging - Proceedings of SPIE |
| Volume | 5690 |
| DOIs | |
| State | Published - Jul 21 2005 |
| Externally published | Yes |
| Event | Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX - San Jose, CA, United States Duration: Jan 23 2005 → Jan 26 2005 |
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Keywords
- Adaptive optics
- Eye
- Optical coherence tomography
- Photoreceptors
- Retina
- Spectroscopy
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomaterials
- Radiology Nuclear Medicine and imaging
Cite this
Single shot retinal imaging with AO spectral OCT. / Zhang, Yan; Rha, Jungtae; Jonnal, Ravi; Miller, Donald T.
In: Progress in Biomedical Optics and Imaging - Proceedings of SPIE, Vol. 5690, 95, 21.07.2005, p. 548-555.Research output: Contribution to journal › Conference article
}
TY - JOUR
T1 - Single shot retinal imaging with AO spectral OCT
AU - Zhang, Yan
AU - Rha, Jungtae
AU - Jonnal, Ravi
AU - Miller, Donald T.
PY - 2005/7/21
Y1 - 2005/7/21
N2 - We demonstrate for the first time an adaptive optics (AO) spectral OCT retina camera that acquires with unprecedented 3D resolution (2.9 μm lateral; 5.5 μm axial) single shot B-scans of the living human retina. The camera centers on a Michelson interferometer that consists of a superluminescent diode for line illuminating the subject's retinal; voice coil translator for controlling the optical path length of the reference channel; and an imaging spectrometer that is cascaded with a 12-bit area CCD array. The imaging spectrometer was designed with negligible off-axis aberrations and was constructed from stock optical components. AO was integrated into the detector channel of the interferometer and dynamically compensated for most of the ocular aberration across a 6 mm pupil. Short bursts of B-scans, with 100 A-scans each, were successfully acquired at 1 msec intervals. Camera sensitivity was found sufficient to detect reflections from all major retinal layers. Individual outer segments of photoreceptors at different retinal eccentricities were observed in vivo. Periodicity of the outer segments matched cone spacing as measured from AO flood illuminated images of the same patches of retina.
AB - We demonstrate for the first time an adaptive optics (AO) spectral OCT retina camera that acquires with unprecedented 3D resolution (2.9 μm lateral; 5.5 μm axial) single shot B-scans of the living human retina. The camera centers on a Michelson interferometer that consists of a superluminescent diode for line illuminating the subject's retinal; voice coil translator for controlling the optical path length of the reference channel; and an imaging spectrometer that is cascaded with a 12-bit area CCD array. The imaging spectrometer was designed with negligible off-axis aberrations and was constructed from stock optical components. AO was integrated into the detector channel of the interferometer and dynamically compensated for most of the ocular aberration across a 6 mm pupil. Short bursts of B-scans, with 100 A-scans each, were successfully acquired at 1 msec intervals. Camera sensitivity was found sufficient to detect reflections from all major retinal layers. Individual outer segments of photoreceptors at different retinal eccentricities were observed in vivo. Periodicity of the outer segments matched cone spacing as measured from AO flood illuminated images of the same patches of retina.
KW - Adaptive optics
KW - Eye
KW - Optical coherence tomography
KW - Photoreceptors
KW - Retina
KW - Spectroscopy
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U2 - 10.1117/12.592831
DO - 10.1117/12.592831
M3 - Conference article
VL - 5690
SP - 548
EP - 555
JO - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
JF - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
SN - 1605-7422
M1 - 95
ER -