Abstract
Coherent diffractive imaging of single particles using the single-shot "diffract and destroy" approach with an x-ray free electron laser (FEL) was recently demonstrated. A high-resolution low-noise coherent diffraction pattern, representative of the object before it turns into a plasma and explodes, results from the interaction of the FEL with the particle. Iterative phase retrieval algorithms are used to reconstruct two-dimensional projection images of the object from the recorded intensities alone. Here we describe the first single-shot diffraction data set that mimics the data proposed for obtaining 3D structure from identical particles. Ellipsoidal iron oxide nanoparticles (250nm×50nm) were aerosolized and injected through an aerodynamic lens stack into a soft x-ray FEL. Particle orientation was not controlled with this injection method. We observed that, at the instant the x-ray pulse interacts with the particle, a snapshot of the particle's orientation is encoded in the diffraction pattern. The results give credence to one of the technical concepts of imaging individual nanometer and subnanometer-sized objects such as single molecules or larger clusters of molecules using hard x-ray FELs and will be used to help develop robust algorithms for determining particle orientations and 3D structure.
| Original language | English (US) |
|---|---|
| Article number | 094701 |
| Journal | Physical Review Special Topics - Accelerators and Beams |
| Volume | 13 |
| Issue number | 9 |
| DOIs | |
| State | Published - Oct 8 2010 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Nuclear and High Energy Physics
- Physics and Astronomy (miscellaneous)
- Surfaces and Interfaces
Cite this
Single-shot femtosecond x-ray diffraction from randomly oriented ellipsoidal nanoparticles. / Bogan, M. J.; Boutet, S.; Barty, A.; Benner, W. H.; Frank, Matthias; Lomb, L.; Shoeman, R.; Starodub, D.; Seibert, M. M.; Hau-Riege, S. P.; Woods, B.; Decorwin-Martin, P.; Bajt, S.; Schulz, J.; Rohner, U.; Iwan, B.; Timneanu, N.; Marchesini, S.; Schlichting, I.; Hajdu, J.; Chapman, H. N.
In: Physical Review Special Topics - Accelerators and Beams, Vol. 13, No. 9, 094701, 08.10.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Single-shot femtosecond x-ray diffraction from randomly oriented ellipsoidal nanoparticles
AU - Bogan, M. J.
AU - Boutet, S.
AU - Barty, A.
AU - Benner, W. H.
AU - Frank, Matthias
AU - Lomb, L.
AU - Shoeman, R.
AU - Starodub, D.
AU - Seibert, M. M.
AU - Hau-Riege, S. P.
AU - Woods, B.
AU - Decorwin-Martin, P.
AU - Bajt, S.
AU - Schulz, J.
AU - Rohner, U.
AU - Iwan, B.
AU - Timneanu, N.
AU - Marchesini, S.
AU - Schlichting, I.
AU - Hajdu, J.
AU - Chapman, H. N.
PY - 2010/10/8
Y1 - 2010/10/8
N2 - Coherent diffractive imaging of single particles using the single-shot "diffract and destroy" approach with an x-ray free electron laser (FEL) was recently demonstrated. A high-resolution low-noise coherent diffraction pattern, representative of the object before it turns into a plasma and explodes, results from the interaction of the FEL with the particle. Iterative phase retrieval algorithms are used to reconstruct two-dimensional projection images of the object from the recorded intensities alone. Here we describe the first single-shot diffraction data set that mimics the data proposed for obtaining 3D structure from identical particles. Ellipsoidal iron oxide nanoparticles (250nm×50nm) were aerosolized and injected through an aerodynamic lens stack into a soft x-ray FEL. Particle orientation was not controlled with this injection method. We observed that, at the instant the x-ray pulse interacts with the particle, a snapshot of the particle's orientation is encoded in the diffraction pattern. The results give credence to one of the technical concepts of imaging individual nanometer and subnanometer-sized objects such as single molecules or larger clusters of molecules using hard x-ray FELs and will be used to help develop robust algorithms for determining particle orientations and 3D structure.
AB - Coherent diffractive imaging of single particles using the single-shot "diffract and destroy" approach with an x-ray free electron laser (FEL) was recently demonstrated. A high-resolution low-noise coherent diffraction pattern, representative of the object before it turns into a plasma and explodes, results from the interaction of the FEL with the particle. Iterative phase retrieval algorithms are used to reconstruct two-dimensional projection images of the object from the recorded intensities alone. Here we describe the first single-shot diffraction data set that mimics the data proposed for obtaining 3D structure from identical particles. Ellipsoidal iron oxide nanoparticles (250nm×50nm) were aerosolized and injected through an aerodynamic lens stack into a soft x-ray FEL. Particle orientation was not controlled with this injection method. We observed that, at the instant the x-ray pulse interacts with the particle, a snapshot of the particle's orientation is encoded in the diffraction pattern. The results give credence to one of the technical concepts of imaging individual nanometer and subnanometer-sized objects such as single molecules or larger clusters of molecules using hard x-ray FELs and will be used to help develop robust algorithms for determining particle orientations and 3D structure.
UR - http://www.scopus.com/inward/record.url?scp=77957328882&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77957328882&partnerID=8YFLogxK
U2 - 10.1103/PhysRevSTAB.13.094701
DO - 10.1103/PhysRevSTAB.13.094701
M3 - Article
AN - SCOPUS:77957328882
VL - 13
JO - Physical Review Special Topics - Accelerators and Beams
JF - Physical Review Special Topics - Accelerators and Beams
SN - 1098-4402
IS - 9
M1 - 094701
ER -