Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells

Matthias Frank; Eric E. Gard; Herbert J. Tobias; Kristl L. Adams; Michael J. Bogan; Keith R. Coffee; George R. Farquar; David P. Fergenson; Sue I. Martin; Maurice Pitesky; Vincent J. Riot; Abneesh Srivastava; Paul T. Steele; Audrey M. Williams

doi:10.1021/bk-2011-1065.ch010

Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells

Matthias Frank, Eric E. Gard, Herbert J. Tobias, Kristl L. Adams, Michael J. Bogan, Keith R. Coffee, George R. Farquar, David P. Fergenson, Sue I. Martin, Maurice Pitesky, Vincent J. Riot, Abneesh Srivastava, Paul T. Steele, Audrey M. Williams

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

8 Scopus citations

Abstract

At Lawrence Livermore National Laboratory, we have developed a single-particle aerosol mass spectrometry (SPAMS) system that can rapidly analyze individual micrometer-sized biological aerosol particles or cells that are sampled directly from air or a lab-generated aerosol into a mass spectrometer. As particles enter the SPAMS system, their aerodynamic size and fluorescence properties are measured before mass spectra from both positive and negative ions created by matrix-free laser desorption and ionization are recorded. All the correlated data obtained from a particle can be analyzed and classified in real-time. The SPAMS system is capable of discriminating, particle by particle, between bacterial spores, vegetative cells and other biological and non-biological background materials using the mass fingerprints obtained from those particles. In addition, selected species of bacteria can be discriminated from each other with this method. Here we describe the overall architecture of the SPAMS system and the related algorithms. We present selected results from applying the SPAMS technique to the analysis of biological agent simulants and single cells. We also describe results from first proof-of-concept experiments using SPAMS for the rapid screening of human effluents for tuberculosis. Lastly, we present results from a field study in a large airport using SPAMS to assess biological content in ambient aerosol.

Original language	English (US)
Title of host publication	Rapid Characterization of Microorganisms by Mass Spectrometry
Publisher	American Chemical Society
Pages	161-196
Number of pages	36
ISBN (Print)	9780841226128
DOIs	https://doi.org/10.1021/bk-2011-1065.ch010
State	Published - Jan 1 2011
Externally published	Yes

Publication series

Name	ACS Symposium Series
Volume	1065
ISSN (Print)	0097-6156
ISSN (Electronic)	1947-5918

ASJC Scopus subject areas

Chemistry(all)
Chemical Engineering(all)

Access to Document

10.1021/bk-2011-1065.ch010

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Frank, M., Gard, E. E., Tobias, H. J., Adams, K. L., Bogan, M. J., Coffee, K. R., Farquar, G. R., Fergenson, D. P., Martin, S. I., Pitesky, M., Riot, V. J., Srivastava, A., Steele, P. T., & Williams, A. M. (2011). Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells. In Rapid Characterization of Microorganisms by Mass Spectrometry (pp. 161-196). (ACS Symposium Series; Vol. 1065). American Chemical Society. https://doi.org/10.1021/bk-2011-1065.ch010

Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells. / Frank, Matthias; Gard, Eric E.; Tobias, Herbert J.; Adams, Kristl L.; Bogan, Michael J.; Coffee, Keith R.; Farquar, George R.; Fergenson, David P.; Martin, Sue I.; Pitesky, Maurice; Riot, Vincent J.; Srivastava, Abneesh; Steele, Paul T.; Williams, Audrey M.

Rapid Characterization of Microorganisms by Mass Spectrometry. American Chemical Society, 2011. p. 161-196 (ACS Symposium Series; Vol. 1065).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Frank, M, Gard, EE, Tobias, HJ, Adams, KL, Bogan, MJ, Coffee, KR, Farquar, GR, Fergenson, DP, Martin, SI, Pitesky, M, Riot, VJ, Srivastava, A, Steele, PT & Williams, AM 2011, Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells. in Rapid Characterization of Microorganisms by Mass Spectrometry. ACS Symposium Series, vol. 1065, American Chemical Society, pp. 161-196. https://doi.org/10.1021/bk-2011-1065.ch010

Frank M, Gard EE, Tobias HJ, Adams KL, Bogan MJ, Coffee KR et al. Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells. In Rapid Characterization of Microorganisms by Mass Spectrometry. American Chemical Society. 2011. p. 161-196. (ACS Symposium Series). https://doi.org/10.1021/bk-2011-1065.ch010

Frank, Matthias ; Gard, Eric E. ; Tobias, Herbert J. ; Adams, Kristl L. ; Bogan, Michael J. ; Coffee, Keith R. ; Farquar, George R. ; Fergenson, David P. ; Martin, Sue I. ; Pitesky, Maurice ; Riot, Vincent J. ; Srivastava, Abneesh ; Steele, Paul T. ; Williams, Audrey M. / Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells. Rapid Characterization of Microorganisms by Mass Spectrometry. American Chemical Society, 2011. pp. 161-196 (ACS Symposium Series).

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abstract = "At Lawrence Livermore National Laboratory, we have developed a single-particle aerosol mass spectrometry (SPAMS) system that can rapidly analyze individual micrometer-sized biological aerosol particles or cells that are sampled directly from air or a lab-generated aerosol into a mass spectrometer. As particles enter the SPAMS system, their aerodynamic size and fluorescence properties are measured before mass spectra from both positive and negative ions created by matrix-free laser desorption and ionization are recorded. All the correlated data obtained from a particle can be analyzed and classified in real-time. The SPAMS system is capable of discriminating, particle by particle, between bacterial spores, vegetative cells and other biological and non-biological background materials using the mass fingerprints obtained from those particles. In addition, selected species of bacteria can be discriminated from each other with this method. Here we describe the overall architecture of the SPAMS system and the related algorithms. We present selected results from applying the SPAMS technique to the analysis of biological agent simulants and single cells. We also describe results from first proof-of-concept experiments using SPAMS for the rapid screening of human effluents for tuberculosis. Lastly, we present results from a field study in a large airport using SPAMS to assess biological content in ambient aerosol.",

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Single-Particle Aerosol Mass Spectrometry (SPAMS) for high-throughput and rapid analysis of biological aerosols and single cells

Abstract

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