Single nucleotide polymorphisms alter kinase anchoring and the subcellular targeting of A-kinase anchoring proteins

F. Donelson Smith, Mitchell H. Omar, Patrick J. Nygren, Joseph Soughayer, Naoto Hoshi, Ho Tak Lau, Calvin G. Snyder, Tess C. Branon, Debapriya Ghosh, Lorene K. Langeberg, Alice Y. Ting, Luis Fernando Santana, Shao En Ong, Manuel F Navedo, John D. Scott

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A-kinase anchoring proteins (AKAPs) shape second-messenger signaling responses by constraining protein kinase A (PKA) at precise intracellular locations. A defining feature of AKAPs is a helical region that binds to regulatory subunits (RII) of PKA. Mining patient-derived databases has identified 42 nonsynonymous SNPs in the PKA-anchoring helices of five AKAPs. Solid-phase RII binding assays confirmed that 21 of these amino acid substitutions disrupt PKA anchoring. The most deleterious side-chain modifications are situated toward C-termini of AKAP helices. More extensive analysis was conducted on a valine-to-methionine variant in the PKA-anchoring helix of AKAP18. Molecular modeling indicates that additional density provided by methionine at position 282 in the AKAP18γ isoform deflects the pitch of the helical anchoring surface outward by 6.6°. Fluorescence polarization measurements show that this subtle topological change reduces RII-binding affinity 8.8-fold and impairs cAMP responsive potentiation of L-type Ca2+ currents in situ. Live-cell imaging of AKAP18γ V282M-GFP adducts led to the unexpected discovery that loss of PKA anchoring promotes nuclear accumulation of this polymorphic variant. Targeting proceeds via a mechanism whereby association with the PKA holoenzyme masks a polybasic nuclear localization signal on the anchoring protein. This led to the discovery of AKAP18e: an exclusively nuclear isoform that lacks a PKA-anchoring helix. Enzyme-mediated proximity-proteomics reveal that compartment-selective variants of AKAP18 associate with distinct binding partners. Thus, naturally occurring PKA-anchoring-defective AKAP variants not only perturb dissemination of local second-messenger responses, but also may influence the intracellular distribution of certain AKAP18 isoforms.

Original languageEnglish (US)
Pages (from-to)E11465-E11474
JournalProceedings of the National Academy of Sciences of the United States of America
Volume115
Issue number49
DOIs
StatePublished - Dec 4 2018

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Cyclic AMP-Dependent Protein Kinases
Protein Kinases
Single Nucleotide Polymorphism
Phosphotransferases
Protein Isoforms
Second Messenger Systems
Methionine
Nuclear Localization Signals
Holoenzymes
Fluorescence Polarization
Valine
Amino Acid Substitution
Masks
Proteomics
Databases
Enzymes

Keywords

  • AKAPs
  • Kinase anchoring
  • Nucleus
  • PKA
  • Proximity labeling

ASJC Scopus subject areas

  • General

Cite this

Single nucleotide polymorphisms alter kinase anchoring and the subcellular targeting of A-kinase anchoring proteins. / Donelson Smith, F.; Omar, Mitchell H.; Nygren, Patrick J.; Soughayer, Joseph; Hoshi, Naoto; Lau, Ho Tak; Snyder, Calvin G.; Branon, Tess C.; Ghosh, Debapriya; Langeberg, Lorene K.; Ting, Alice Y.; Santana, Luis Fernando; Ong, Shao En; Navedo, Manuel F; Scott, John D.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 115, No. 49, 04.12.2018, p. E11465-E11474.

Research output: Contribution to journalArticle

Donelson Smith, F, Omar, MH, Nygren, PJ, Soughayer, J, Hoshi, N, Lau, HT, Snyder, CG, Branon, TC, Ghosh, D, Langeberg, LK, Ting, AY, Santana, LF, Ong, SE, Navedo, MF & Scott, JD 2018, 'Single nucleotide polymorphisms alter kinase anchoring and the subcellular targeting of A-kinase anchoring proteins', Proceedings of the National Academy of Sciences of the United States of America, vol. 115, no. 49, pp. E11465-E11474. https://doi.org/10.1073/pnas.1816614115
Donelson Smith, F. ; Omar, Mitchell H. ; Nygren, Patrick J. ; Soughayer, Joseph ; Hoshi, Naoto ; Lau, Ho Tak ; Snyder, Calvin G. ; Branon, Tess C. ; Ghosh, Debapriya ; Langeberg, Lorene K. ; Ting, Alice Y. ; Santana, Luis Fernando ; Ong, Shao En ; Navedo, Manuel F ; Scott, John D. / Single nucleotide polymorphisms alter kinase anchoring and the subcellular targeting of A-kinase anchoring proteins. In: Proceedings of the National Academy of Sciences of the United States of America. 2018 ; Vol. 115, No. 49. pp. E11465-E11474.
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abstract = "A-kinase anchoring proteins (AKAPs) shape second-messenger signaling responses by constraining protein kinase A (PKA) at precise intracellular locations. A defining feature of AKAPs is a helical region that binds to regulatory subunits (RII) of PKA. Mining patient-derived databases has identified 42 nonsynonymous SNPs in the PKA-anchoring helices of five AKAPs. Solid-phase RII binding assays confirmed that 21 of these amino acid substitutions disrupt PKA anchoring. The most deleterious side-chain modifications are situated toward C-termini of AKAP helices. More extensive analysis was conducted on a valine-to-methionine variant in the PKA-anchoring helix of AKAP18. Molecular modeling indicates that additional density provided by methionine at position 282 in the AKAP18γ isoform deflects the pitch of the helical anchoring surface outward by 6.6°. Fluorescence polarization measurements show that this subtle topological change reduces RII-binding affinity 8.8-fold and impairs cAMP responsive potentiation of L-type Ca2+ currents in situ. Live-cell imaging of AKAP18γ V282M-GFP adducts led to the unexpected discovery that loss of PKA anchoring promotes nuclear accumulation of this polymorphic variant. Targeting proceeds via a mechanism whereby association with the PKA holoenzyme masks a polybasic nuclear localization signal on the anchoring protein. This led to the discovery of AKAP18e: an exclusively nuclear isoform that lacks a PKA-anchoring helix. Enzyme-mediated proximity-proteomics reveal that compartment-selective variants of AKAP18 associate with distinct binding partners. Thus, naturally occurring PKA-anchoring-defective AKAP variants not only perturb dissemination of local second-messenger responses, but also may influence the intracellular distribution of certain AKAP18 isoforms.",
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AU - Soughayer, Joseph

AU - Hoshi, Naoto

AU - Lau, Ho Tak

AU - Snyder, Calvin G.

AU - Branon, Tess C.

AU - Ghosh, Debapriya

AU - Langeberg, Lorene K.

AU - Ting, Alice Y.

AU - Santana, Luis Fernando

AU - Ong, Shao En

AU - Navedo, Manuel F

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