Single molecule pulsed interleaved excitation fluorescence resonance energy transfer (PIE-FRET) inside nanometer-scale apertures at biologically relevant concentration

Samantha Fore; Thomas R Huser; Yin Yuen; Lambertus Hesselink

Single molecule pulsed interleaved excitation fluorescence resonance energy transfer (PIE-FRET) inside nanometer-scale apertures at biologically relevant concentration

Samantha Fore, Thomas R Huser, Yin Yuen, Lambertus Hesselink

Endocrinology, Diabetes, and Metabolism

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

PIE-FRET offers significant advantages over conventional single molecule FRET techniques, but it still requires the dilution of samples to biologically low concentrations. Here, we present FRET measurements inside nm-sized apertures at ~1000 times higher concentration.

Original language	English (US)
Title of host publication	Optics InfoBase Conference Papers
Publisher	Optical Society of America
ISBN (Print)	1557528349, 9781557528346
State	Published - 2007
Event	Conference on Lasers and Electro-Optics, CLEO 2007 - Baltimore, MD, United States Duration: May 6 2007 → May 6 2007

Other

Other	Conference on Lasers and Electro-Optics, CLEO 2007
Country	United States
City	Baltimore, MD
Period	5/6/07 → 5/6/07

ASJC Scopus subject areas

Instrumentation
Atomic and Molecular Physics, and Optics

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Single molecule pulsed interleaved excitation fluorescence resonance energy transfer (PIE-FRET) inside nanometer-scale apertures at biologically relevant concentration. / Fore, Samantha; Huser, Thomas R; Yuen, Yin; Hesselink, Lambertus.

Optics InfoBase Conference Papers. Optical Society of America, 2007.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Fore, S, Huser, TR, Yuen, Y & Hesselink, L 2007, Single molecule pulsed interleaved excitation fluorescence resonance energy transfer (PIE-FRET) inside nanometer-scale apertures at biologically relevant concentration. in Optics InfoBase Conference Papers. Optical Society of America, Conference on Lasers and Electro-Optics, CLEO 2007, Baltimore, MD, United States, 5/6/07.

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title = "Single molecule pulsed interleaved excitation fluorescence resonance energy transfer (PIE-FRET) inside nanometer-scale apertures at biologically relevant concentration",

abstract = "PIE-FRET offers significant advantages over conventional single molecule FRET techniques, but it still requires the dilution of samples to biologically low concentrations. Here, we present FRET measurements inside nm-sized apertures at ~1000 times higher concentration.",

author = "Samantha Fore and Huser, {Thomas R} and Yin Yuen and Lambertus Hesselink",

year = "2007",

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PY - 2007

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SN - 9781557528346

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