Single molecule pulsed interleaved excitation fluorescence resonance energy transfer (PIE-FRET) inside nanometer-scale apertures at biologically relevant concentration

Samantha Fore, Thomas R Huser, Yin Yuen, Lambertus Hesselink

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

PIE-FRET offers significant advantages over conventional single molecule FRET techniques, but it still requires the dilution of samples to biologically low concentrations. Here, we present FRET measurements inside nm-sized apertures at ~1000 times higher concentration.

Original languageEnglish (US)
Title of host publicationOptics InfoBase Conference Papers
PublisherOptical Society of America
ISBN (Print)1557528349, 9781557528346
StatePublished - 2007
EventConference on Lasers and Electro-Optics, CLEO 2007 - Baltimore, MD, United States
Duration: May 6 2007May 6 2007

Other

OtherConference on Lasers and Electro-Optics, CLEO 2007
CountryUnited States
CityBaltimore, MD
Period5/6/075/6/07

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Fore, S., Huser, T. R., Yuen, Y., & Hesselink, L. (2007). Single molecule pulsed interleaved excitation fluorescence resonance energy transfer (PIE-FRET) inside nanometer-scale apertures at biologically relevant concentration. In Optics InfoBase Conference Papers Optical Society of America.