Single-molecule imaging brings Rad51 nucleoprotein filaments into focus

Anthony L. Forget, Stephen C. Kowalczykowski

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

The Rad51 protein is essential for DNA repair by homologous recombination. After DNA damage, Rad51 localizes to nuclear foci that represent sites of DNA repair in vivo. In vitro, Rad51 self-assembles on single- or double-stranded DNA to form a nucleoprotein filament. Recently, the merging of innovative single-molecule techniques with ensemble methods has provided unique insights into the dynamic nature of this filament and its cellular function. The assembly and disassembly of Rad51 nucleoprotein filaments is seen to be regulated by recombination accessory proteins. In this regard, the BRC repeats of the BRCA2 protein were shown to modulate the DNA binding selectivity of Rad51. Furthermore, single-molecule studies explained the need for a DNA translocase, Rad54 protein, in the disassembly of Rad51 double-stranded DNA filaments.

Original languageEnglish (US)
Pages (from-to)269-276
Number of pages8
JournalTrends in Cell Biology
Volume20
Issue number5
DOIs
StatePublished - May 2010

ASJC Scopus subject areas

  • Cell Biology

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