Single cell wound generates electric current circuit and cell membrane potential variations that requires calcium influx

Guillaume Luxardi, Brian Reid, Pauline Maillard, Min Zhao

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Breaching of the cell membrane is one of the earliest and most common causes of cell injury, tissue damage, and disease. If the compromise in cell membrane is not repaired quickly, irreversible cell damage, cell death and defective organ functions will result. It is therefore fundamentally important to efficiently repair damage to the cell membrane. While the molecular aspects of single cell wound healing are starting to be deciphered, its bio-physical counterpart has been poorly investigated. Using Xenopus laevis oocytes as a model for single cell wound healing, we describe the temporal and spatial dynamics of the wound electric current circuitry and the temporal dynamics of cell membrane potential variation. In addition, we show the role of calcium influx in controlling electric current circuitry and cell membrane potential variations. (i) Upon wounding a single cell: an inward electric current appears at the wound center while an outward electric current is observed at its sides, illustrating the wound electric current circuitry; the cell membrane is depolarized; calcium flows into the cell. (ii) During cell membrane re-sealing: the wound center current density is maintained for a few minutes before decreasing; the cell membrane gradually re-polarizes; calcium flow into the cell drops. (iii) In conclusion, calcium influx is required for the formation and maintenance of the wound electric current circuitry, for cell membrane re-polarization and for wound healing.

Original languageEnglish (US)
Pages (from-to)662-672
Number of pages11
JournalIntegrative biology : quantitative biosciences from nano to macro
Volume6
Issue number7
DOIs
StatePublished - Jul 24 2014

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Electric currents
Cell membranes
Membrane Potentials
Cell Membrane
Calcium
Networks (circuits)
Wounds and Injuries
Wound Healing
Electric batteries
Xenopus laevis
Cell death
Oocytes
Repair
Cell Death
Current density
Cells
Maintenance
Polarization
Tissue

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Single cell wound generates electric current circuit and cell membrane potential variations that requires calcium influx. / Luxardi, Guillaume; Reid, Brian; Maillard, Pauline; Zhao, Min.

In: Integrative biology : quantitative biosciences from nano to macro, Vol. 6, No. 7, 24.07.2014, p. 662-672.

Research output: Contribution to journalArticle

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