@inbook{f05b2b98812244a6b510292d8436b747,
title = "Single-cell imaging of ERK signaling using fluorescent biosensors",
abstract = "Single-cell analysis of the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) provides a means to perform highly detailed kinetic studies, assess heterogeneity between cells, and distinguish the subcellular localization of ERK activity. We describe here the methods needed to perform such measurements in a cell type of the investigator{\textquoteright}s choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells.",
keywords = "ERK, FRET, Live-cell microscopy, MAPK, Single cell, Translocation",
author = "Michael Pargett and Gillies, {Taryn E.} and Teragawa, {Carolyn K.} and Breanne Sparta and John Albeck",
year = "2017",
month = jan,
day = "1",
doi = "10.1007/978-1-4939-7154-1_3",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "35--59",
booktitle = "Methods in Molecular Biology",
}