Single-cell imaging of ERK signaling using fluorescent biosensors

Michael Pargett, Taryn E. Gillies, Carolyn K. Teragawa, Breanne Sparta, John Albeck

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

Single-cell analysis of the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) provides a means to perform highly detailed kinetic studies, assess heterogeneity between cells, and distinguish the subcellular localization of ERK activity. We describe here the methods needed to perform such measurements in a cell type of the investigator’s choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-59
Number of pages25
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1636
ISSN (Print)1064-3745

Fingerprint

Extracellular Signal-Regulated MAP Kinases
Biosensing Techniques
Single-Cell Analysis
Mitogen-Activated Protein Kinases
Research Personnel

Keywords

  • ERK
  • FRET
  • Live-cell microscopy
  • MAPK
  • Single cell
  • Translocation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Pargett, M., Gillies, T. E., Teragawa, C. K., Sparta, B., & Albeck, J. (2017). Single-cell imaging of ERK signaling using fluorescent biosensors. In Methods in Molecular Biology (pp. 35-59). (Methods in Molecular Biology; Vol. 1636). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7154-1_3

Single-cell imaging of ERK signaling using fluorescent biosensors. / Pargett, Michael; Gillies, Taryn E.; Teragawa, Carolyn K.; Sparta, Breanne; Albeck, John.

Methods in Molecular Biology. Humana Press Inc., 2017. p. 35-59 (Methods in Molecular Biology; Vol. 1636).

Research output: Chapter in Book/Report/Conference proceedingChapter

Pargett, M, Gillies, TE, Teragawa, CK, Sparta, B & Albeck, J 2017, Single-cell imaging of ERK signaling using fluorescent biosensors. in Methods in Molecular Biology. Methods in Molecular Biology, vol. 1636, Humana Press Inc., pp. 35-59. https://doi.org/10.1007/978-1-4939-7154-1_3
Pargett M, Gillies TE, Teragawa CK, Sparta B, Albeck J. Single-cell imaging of ERK signaling using fluorescent biosensors. In Methods in Molecular Biology. Humana Press Inc. 2017. p. 35-59. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-7154-1_3
Pargett, Michael ; Gillies, Taryn E. ; Teragawa, Carolyn K. ; Sparta, Breanne ; Albeck, John. / Single-cell imaging of ERK signaling using fluorescent biosensors. Methods in Molecular Biology. Humana Press Inc., 2017. pp. 35-59 (Methods in Molecular Biology).
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