Single-cell imaging of ERK signaling using fluorescent biosensors

Michael Pargett, Taryn E. Gillies, Carolyn K. Teragawa, Breanne Sparta, John Albeck

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Single-cell analysis of the mitogen-activated protein kinase (MAPK) extracellular signal-regulated kinase (ERK) provides a means to perform highly detailed kinetic studies, assess heterogeneity between cells, and distinguish the subcellular localization of ERK activity. We describe here the methods needed to perform such measurements in a cell type of the investigator’s choosing. We discuss the selection of appropriate reporters and provide detailed methods for stably introducing reporters, collecting live-cell data, and automatically extracting quantitative information from individual cells.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages35-59
Number of pages25
DOIs
StatePublished - Jan 1 2017

Publication series

NameMethods in Molecular Biology
Volume1636
ISSN (Print)1064-3745

Keywords

  • ERK
  • FRET
  • Live-cell microscopy
  • MAPK
  • Single cell
  • Translocation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Pargett, M., Gillies, T. E., Teragawa, C. K., Sparta, B., & Albeck, J. (2017). Single-cell imaging of ERK signaling using fluorescent biosensors. In Methods in Molecular Biology (pp. 35-59). (Methods in Molecular Biology; Vol. 1636). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7154-1_3