Single-cell adhesion tests against functionalized microspheres arrayed on AFM cantilevers confirm heterophilic E- and N-cadherin binding

Chawin Ounkomol, Soichiro Yamada, Volkmar Heinrich

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Weassess the cross-reactivity of both cellular as well as recombinant E- and N-cadherins using functionalized bead arrays assembled on atomic-force-microscope cantilevers. This new approach builds upon and enhances the utility of a recently developed force probe that integrates a custom-built, horizontal atomic force microscope with micropipette manipulation. It enables us to test multiple biomolecular interactions of the same cell in a swift sequential or cyclic manner and thus to resolve subtle differences between individual interactions that otherwise would be obscured by cell-cell baseline variability. For each cell, we contrast heterophilic E:N-cadherin binding with the respectiv homophilic bonds and with a suitable control. Clarifying previous literature reports, we establish that specific bonds between E- and N-cadherins form readily, albeit less frequently than homophilic bonds of either cadherin. We support this assessment with a rough estimate of the ratio of on-rate constants of E/N-cadherin binding.

Original languageEnglish (US)
JournalBiophysical Journal
Volume99
Issue number12
DOIs
StatePublished - Dec 15 2010

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Cadherins
Microspheres
Cell Adhesion
Individuality
Cell Communication

ASJC Scopus subject areas

  • Biophysics

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Single-cell adhesion tests against functionalized microspheres arrayed on AFM cantilevers confirm heterophilic E- and N-cadherin binding. / Ounkomol, Chawin; Yamada, Soichiro; Heinrich, Volkmar.

In: Biophysical Journal, Vol. 99, No. 12, 15.12.2010.

Research output: Contribution to journalArticle

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