Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis

Sun Yinghua; Liu Rui; Daniel S. Elson; Christopher W. Hollars; Javier A. Jo; Park Jesung; Sun Yang; Laura Marcu

doi:10.1364/OL.33.000630

Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis

Sun Yinghua, Liu Rui, Daniel S. Elson, Christopher W. Hollars, Javier A. Jo, Park Jesung, Sun Yang, Laura Marcu

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems.

Original language	English (US)
Pages (from-to)	630-632
Number of pages	3
Journal	Optics Letters
Volume	33
Issue number	6
DOIs	https://doi.org/10.1364/OL.33.000630
State	Published - Mar 15 2008

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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title = "Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis",

abstract = "A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems.",

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T1 - Simultaneous time- and wavelength-resolved fluorescence spectroscopy for near real-time tissue diagnosis

AU - Yinghua, Sun

AU - Rui, Liu

AU - Elson, Daniel S.

AU - Hollars, Christopher W.

AU - Jo, Javier A.

AU - Jesung, Park

AU - Yang, Sun

AU - Marcu, Laura

PY - 2008/3/15

Y1 - 2008/3/15

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AB - A novel fiber-optic-based method for simultaneous time- and wavelength-resolved fluorescence spectroscopy for the rapid diagnosis of diseased tissue is demonstrated. By combining multiple bandpass and dichroic filters (405/40, 460/50, and 550/50) with different lengths of optical fiber (1, 10, and 19 m) acting as an optical delay this system enables the near real-time acquisition and characterization of time-resolved fluorescence spectra using a single detector and excitation input. The recording of multiple fluorescence response pulses at selected wavelengths can be completed in hundreds of nanoseconds, which provides the capability of a real-time characterization of biological systems.

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