Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber

Benjamin E. Sherlock, Jennifer E. Phipps, Julien Bec, Laura Marcu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.

Original languageEnglish (US)
Pages (from-to)3753-3756
Number of pages4
JournalOptics Letters
Volume42
Issue number19
DOIs
StatePublished - Oct 1 2017

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tomography
life (durability)
fluorescence
fibers
platforms
histology
arteries
scanning

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber. / Sherlock, Benjamin E.; Phipps, Jennifer E.; Bec, Julien; Marcu, Laura.

In: Optics Letters, Vol. 42, No. 19, 01.10.2017, p. 3753-3756.

Research output: Contribution to journalArticle

Sherlock, Benjamin E. ; Phipps, Jennifer E. ; Bec, Julien ; Marcu, Laura. / Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber. In: Optics Letters. 2017 ; Vol. 42, No. 19. pp. 3753-3756.
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