Abstract
We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3753-3756 |
| Number of pages | 4 |
| Journal | Optics Letters |
| Volume | 42 |
| Issue number | 19 |
| DOIs | |
| State | Published - Oct 1 2017 |
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ASJC Scopus subject areas
- Atomic and Molecular Physics, and Optics
Cite this
Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber. / Sherlock, Benjamin E.; Phipps, Jennifer E.; Bec, Julien; Marcu, Laura.
In: Optics Letters, Vol. 42, No. 19, 01.10.2017, p. 3753-3756.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber
AU - Sherlock, Benjamin E.
AU - Phipps, Jennifer E.
AU - Bec, Julien
AU - Marcu, Laura
PY - 2017/10/1
Y1 - 2017/10/1
N2 - We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.
AB - We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.
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UR - http://www.scopus.com/inward/citedby.url?scp=85030168111&partnerID=8YFLogxK
U2 - 10.1364/OL.42.003753
DO - 10.1364/OL.42.003753
M3 - Article
AN - SCOPUS:85030168111
VL - 42
SP - 3753
EP - 3756
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 19
ER -