Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber

Benjamin E. Sherlock; Jennifer E. Phipps; Julien Bec; Laura Marcu

doi:10.1364/OL.42.003753

Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber

Benjamin E. Sherlock, Jennifer E. Phipps, Julien Bec, Laura Marcu

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.

Original language	English (US)
Pages (from-to)	3753-3756
Number of pages	4
Journal	Optics Letters
Volume	42
Issue number	19
DOIs	https://doi.org/10.1364/OL.42.003753
State	Published - Oct 1 2017

ASJC Scopus subject areas

Atomic and Molecular Physics, and Optics

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AB - We present a novel fiber-based imaging platform that allows simultaneous fluorescence lifetime imaging (FLIm) and optical coherence tomography (OCT) using a double-clad fiber. This platform acquires co-registered images showing structural and compositional contrast in unlabeled biological samples by scanning the fiber tip across the sample surface. In this Letter, we report a characterization of each modality and show examples of co-registered FLIm and OCT images acquired from a lemon segment and a section of human coronary artery. The close comparison between the combined FLIm and OCT images and a co-registered histology section provides a qualitative validation of the technique and highlights its potential for minimally invasive, multimodal imaging of tissue structure and composition.

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Simultaneous, label-free, multispectral fluorescence lifetime imaging and optical coherence tomography using a double-clad fiber

Abstract

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