Simultaneous haploinsufficiency of Pten and Trp53 tumor suppressor genes accelerates tumorigenesis in a mouse model of prostate cancer

Suzana S. Couto, Mei Cao, Paulo C. Duarte, Whitney Banach-Petrosky, Shunyou Wang, Peter Romanienko, Hong Wu, Robert Cardiff, Cory Abate-Shen, Gerald R. Cunha

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Tumor suppressor gene PTEN is important in the initiation and progression of human prostate carcinoma, whereas the role of TP53 remains controversial. Since Pten/Trp53 double conditional knockout mice show earlier onset and fast progression of prostate cancer when compared to Pten knockout mice, we asked whether heterozygosity of these two tumor suppressor genes was sufficient to accelerate prostatic tumorigenesis. To answer this question we examined prostatic lesion progression of Pten/Trp53 double heterozygous mice and a series of controls such as Pten heterozygous, Pten conditional knockout, Trp53 heterozygous and Trp53 knockout mice. Tissue recombination of adult prostatic epithelium coupled with embryonic rat seminal vesicle mesenchyme was used as a tool to stimulate prostatic epithelial proliferation. In our study, high-grade prostatic intraepithelial neoplasia (PIN) was found with high frequency at 8 weeks post-tissue recombination transplantation. PIN lesions in Pten/Trp53 double heterozygous mice were more severe than those seen in Pten heterozygous alone. Furthermore, morphologic features attributable to Pten or Trp53 loss appeared to be enhanced in double heterozygous tissues. LOH analysis of Pten and Trp53 in genomic DNA collected from high-grade PIN lesions in Pten heterozygous and Pten/Trp53 double heterozygous mice showed an intact wild-type allele for both genes in all samples examined. In conclusion, simultaneous heterozygosity of Pten and Trp53 accelerates prostatic tumorigenesis in this mouse model of prostate cancer independently of loss of heterozygosity of either gene.

Original languageEnglish (US)
Pages (from-to)103-111
Number of pages9
JournalDifferentiation
Volume77
Issue number1
DOIs
StatePublished - Jan 1 2009

Keywords

  • AKT
  • Embryonic mesenchyme
  • Mouse models
  • Pathology
  • Prostate cancer
  • Pten
  • Tissue recombinants
  • Trp53
  • Tumor suppressor genes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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    Couto, S. S., Cao, M., Duarte, P. C., Banach-Petrosky, W., Wang, S., Romanienko, P., Wu, H., Cardiff, R., Abate-Shen, C., & Cunha, G. R. (2009). Simultaneous haploinsufficiency of Pten and Trp53 tumor suppressor genes accelerates tumorigenesis in a mouse model of prostate cancer. Differentiation, 77(1), 103-111. https://doi.org/10.1016/j.diff.2008.09.010