Simultaneous forward and epi-CARS microscopy with a single detector by time-correlated single photon counting

Iwan W. Schie, Tyler Weeks, Gregory P. McNerney, Samantha Fore, Juliana K. Sampson, Sebastian Wachsmann-Hogiu, John C Rutledge, Thomas R Huser

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We present a novel scheme to simultaneously detect coherent anti-Stokes Raman scattering (CARS) microscopy signals in the forward (F) and backward (epi - E) direction with a single avalanche photodiode (APD) detector using time-correlated single photon counting (TCSPC). By installing a mirror at a well-defined distance above the sample the forward-scattered F-CARS signal is reflected back into the microscope objective leading to spatial overlap of the F and E-CARS signals. Due to traveling an additional distance the F-CARS signal is time delayed relative to the E-CARS signal. TCSPC then allows for the two signals to be resolved in the time domain. This results in an efficient, simple, and compact method of CARS signal detection. We demonstrate this technique by analyzing forward and backward CARS signals obtained by imaging living adipocyte cells derived from human mesenchymal stem cells.

Original languageEnglish (US)
Pages (from-to)2168-2175
Number of pages8
JournalOptics Express
Volume16
Issue number3
DOIs
StatePublished - Feb 4 2008

Fingerprint

counting
Raman spectra
microscopy
detectors
photons
time signals
stem cells
signal detection
avalanches
installing
photodiodes
microscopes
mirrors
cells

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

Cite this

Simultaneous forward and epi-CARS microscopy with a single detector by time-correlated single photon counting. / Schie, Iwan W.; Weeks, Tyler; McNerney, Gregory P.; Fore, Samantha; Sampson, Juliana K.; Wachsmann-Hogiu, Sebastian; Rutledge, John C; Huser, Thomas R.

In: Optics Express, Vol. 16, No. 3, 04.02.2008, p. 2168-2175.

Research output: Contribution to journalArticle

Schie, Iwan W. ; Weeks, Tyler ; McNerney, Gregory P. ; Fore, Samantha ; Sampson, Juliana K. ; Wachsmann-Hogiu, Sebastian ; Rutledge, John C ; Huser, Thomas R. / Simultaneous forward and epi-CARS microscopy with a single detector by time-correlated single photon counting. In: Optics Express. 2008 ; Vol. 16, No. 3. pp. 2168-2175.
@article{75f4fc8c93984dab94f87faba0bf249a,
title = "Simultaneous forward and epi-CARS microscopy with a single detector by time-correlated single photon counting",
abstract = "We present a novel scheme to simultaneously detect coherent anti-Stokes Raman scattering (CARS) microscopy signals in the forward (F) and backward (epi - E) direction with a single avalanche photodiode (APD) detector using time-correlated single photon counting (TCSPC). By installing a mirror at a well-defined distance above the sample the forward-scattered F-CARS signal is reflected back into the microscope objective leading to spatial overlap of the F and E-CARS signals. Due to traveling an additional distance the F-CARS signal is time delayed relative to the E-CARS signal. TCSPC then allows for the two signals to be resolved in the time domain. This results in an efficient, simple, and compact method of CARS signal detection. We demonstrate this technique by analyzing forward and backward CARS signals obtained by imaging living adipocyte cells derived from human mesenchymal stem cells.",
author = "Schie, {Iwan W.} and Tyler Weeks and McNerney, {Gregory P.} and Samantha Fore and Sampson, {Juliana K.} and Sebastian Wachsmann-Hogiu and Rutledge, {John C} and Huser, {Thomas R}",
year = "2008",
month = "2",
day = "4",
doi = "10.1364/OE.16.002168",
language = "English (US)",
volume = "16",
pages = "2168--2175",
journal = "Optics Express",
issn = "1094-4087",
publisher = "The Optical Society",
number = "3",

}

TY - JOUR

T1 - Simultaneous forward and epi-CARS microscopy with a single detector by time-correlated single photon counting

AU - Schie, Iwan W.

AU - Weeks, Tyler

AU - McNerney, Gregory P.

AU - Fore, Samantha

AU - Sampson, Juliana K.

AU - Wachsmann-Hogiu, Sebastian

AU - Rutledge, John C

AU - Huser, Thomas R

PY - 2008/2/4

Y1 - 2008/2/4

N2 - We present a novel scheme to simultaneously detect coherent anti-Stokes Raman scattering (CARS) microscopy signals in the forward (F) and backward (epi - E) direction with a single avalanche photodiode (APD) detector using time-correlated single photon counting (TCSPC). By installing a mirror at a well-defined distance above the sample the forward-scattered F-CARS signal is reflected back into the microscope objective leading to spatial overlap of the F and E-CARS signals. Due to traveling an additional distance the F-CARS signal is time delayed relative to the E-CARS signal. TCSPC then allows for the two signals to be resolved in the time domain. This results in an efficient, simple, and compact method of CARS signal detection. We demonstrate this technique by analyzing forward and backward CARS signals obtained by imaging living adipocyte cells derived from human mesenchymal stem cells.

AB - We present a novel scheme to simultaneously detect coherent anti-Stokes Raman scattering (CARS) microscopy signals in the forward (F) and backward (epi - E) direction with a single avalanche photodiode (APD) detector using time-correlated single photon counting (TCSPC). By installing a mirror at a well-defined distance above the sample the forward-scattered F-CARS signal is reflected back into the microscope objective leading to spatial overlap of the F and E-CARS signals. Due to traveling an additional distance the F-CARS signal is time delayed relative to the E-CARS signal. TCSPC then allows for the two signals to be resolved in the time domain. This results in an efficient, simple, and compact method of CARS signal detection. We demonstrate this technique by analyzing forward and backward CARS signals obtained by imaging living adipocyte cells derived from human mesenchymal stem cells.

UR - http://www.scopus.com/inward/record.url?scp=39049147410&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=39049147410&partnerID=8YFLogxK

U2 - 10.1364/OE.16.002168

DO - 10.1364/OE.16.002168

M3 - Article

C2 - 18542297

AN - SCOPUS:39049147410

VL - 16

SP - 2168

EP - 2175

JO - Optics Express

JF - Optics Express

SN - 1094-4087

IS - 3

ER -