Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes

P. Busque, Robert Higgins, S. Sénéchal, R. Marchand, S. Quessy

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)229-238
Number of pages10
JournalVeterinary Microbiology
Volume63
Issue number2-4
DOIs
StatePublished - Oct 1 1998
Externally publishedYes

Fingerprint

Streptococcus suis
Mononuclear Leukocytes
phagocytosis
Phagocytosis
leukocytes
Swine
Fluorescein-5-isothiocyanate
granulocytes
Granulocytes
monocytes
swine
Monocytes
blood
serotypes
Bacteria
avirulent strains
bacteria
Leukocytes
methodology
Serogroup

Keywords

  • Flow cytometry
  • Monocytes
  • Neutrophils
  • Phagocytosis
  • Streptococcus suis

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes. / Busque, P.; Higgins, Robert; Sénéchal, S.; Marchand, R.; Quessy, S.

In: Veterinary Microbiology, Vol. 63, No. 2-4, 01.10.1998, p. 229-238.

Research output: Contribution to journalArticle

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