Abstract
A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 229-238 |
| Number of pages | 10 |
| Journal | Veterinary Microbiology |
| Volume | 63 |
| Issue number | 2-4 |
| DOIs | |
| State | Published - Oct 1 1998 |
| Externally published | Yes |
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Keywords
- Flow cytometry
- Monocytes
- Neutrophils
- Phagocytosis
- Streptococcus suis
ASJC Scopus subject areas
- Microbiology
- veterinary(all)
Cite this
Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes. / Busque, P.; Higgins, Robert; Sénéchal, S.; Marchand, R.; Quessy, S.
In: Veterinary Microbiology, Vol. 63, No. 2-4, 01.10.1998, p. 229-238.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes
AU - Busque, P.
AU - Higgins, Robert
AU - Sénéchal, S.
AU - Marchand, R.
AU - Quessy, S.
PY - 1998/10/1
Y1 - 1998/10/1
N2 - A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.
AB - A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.
KW - Flow cytometry
KW - Monocytes
KW - Neutrophils
KW - Phagocytosis
KW - Streptococcus suis
UR - http://www.scopus.com/inward/record.url?scp=0031726731&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031726731&partnerID=8YFLogxK
U2 - 10.1016/S0378-1135(98)00216-8
DO - 10.1016/S0378-1135(98)00216-8
M3 - Article
C2 - 9851001
AN - SCOPUS:0031726731
VL - 63
SP - 229
EP - 238
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
IS - 2-4
ER -