Abstract
A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 229-238 |
| Number of pages | 10 |
| Journal | Veterinary Microbiology |
| Volume | 63 |
| Issue number | 2-4 |
| DOIs | |
| State | Published - Oct 1 1998 |
| Externally published | Yes |
Keywords
- Flow cytometry
- Monocytes
- Neutrophils
- Phagocytosis
- Streptococcus suis
ASJC Scopus subject areas
- Microbiology
- veterinary(all)
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Busque, P., Higgins, R., Sénéchal, S., Marchand, R., & Quessy, S. (1998). Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes. Veterinary Microbiology, 63(2-4), 229-238. https://doi.org/10.1016/S0378-1135(98)00216-8