Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes

P. Busque; Robert Higgins; S. Sénéchal; R. Marchand; S. Quessy

doi:10.1016/S0378-1135(98)00216-8

Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes

P. Busque, Robert Higgins, S. Sénéchal, R. Marchand, S. Quessy

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	229-238
Number of pages	10
Journal	Veterinary Microbiology
Volume	63
Issue number	2-4
DOIs	https://doi.org/10.1016/S0378-1135(98)00216-8
State	Published - Oct 1 1998
Externally published	Yes

Keywords

Flow cytometry
Monocytes
Neutrophils
Phagocytosis
Streptococcus suis

ASJC Scopus subject areas

Microbiology
veterinary(all)

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title = "Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes",

abstract = "A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.",

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AU - Busque, P.

AU - Higgins, Robert

AU - Sénéchal, S.

AU - Marchand, R.

AU - Quessy, S.

PY - 1998/10/1

Y1 - 1998/10/1

N2 - A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.

AB - A simple flow cytometric method was used to study simultaneously the phagocytosis of Streptococcus suis serotype 2 by polymorphonuclear and mononuclear blood leukocytes from swine and humans. Using this method with a bacteria-to-leukocytes ratio of 10:1 and after 60min of incubation, 80.2±2.8% of swine granulocytes and 77.0±2.8% of swine monocytes were shown to contain FITC-labelled S. suis serotype 2 strain 735. Using the same strain, FITC-labelled bacteria were found in 95.5±3.2% of human granulocytes and in 92.8±3.6% of human monocytes. The phagocytosis rates of avirulent and virulent strains of S. suis were not significantly different. Copyright (C) 1998 Elsevier Science B.V.

KW - Flow cytometry

KW - Monocytes

KW - Neutrophils

KW - Phagocytosis

KW - Streptococcus suis

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Simultaneous flow cytometric measurement of Streptococcus suis phagocytosis by polymorphonuclear and mononuclear blood leukocytes

Abstract

Keywords

ASJC Scopus subject areas

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